Identification of an Endophytic Bacterium XZNUM 033 from Ginkgo biloba L.and Its Physicochemical Characteristic of Anti-sapstain Fungus Activity

LI Chang-gen; CAO Cheng-liang; QIN Sheng; MIAO Qian; SUN Yong; JIANG Ji-hong

The endophytic bacterium XZNUM 033 has a strong inhibitory activity against poplar sapstain pathogen Lasiodiplodia theobromae.The physicochemical property of the fermentation broth was detected. The result showed that fermentation broth with the pH 7.0 had the strongest anti-fungal activity and was stable against heat, light and ultraviolet. According to the morphological observation, gram staining, spore staining, physicochemical property and the phylogenetic analysis of 16S rRNA gene sequence, the strain XZNUM 033 was identified as a known species of the genus Bacillus amyloliquefaciens.

1. Key Laboratory for Biotechnology on Medicinal Plant of Jiangsu Province, Xuzhou Normal University, Xuzhou 221116, Jiangsu, China

Received Date: 2010-02-11

Abstract: The endophytic bacterium XZNUM 033 has a strong inhibitory activity against poplar sapstain pathogen Lasiodiplodia theobromae.The physicochemical property of the fermentation broth was detected. The result showed that fermentation broth with the pH 7.0 had the strongest anti-fungal activity and was stable against heat, light and ultraviolet. According to the morphological observation, gram staining, spore staining, physicochemical property and the phylogenetic analysis of 16S rRNA gene sequence, the strain XZNUM 033 was identified as a known species of the genus Bacillus amyloliquefaciens.

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Reference (17)

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Identification of an Endophytic Bacterium XZNUM 033 from Ginkgo biloba L.and Its Physicochemical Characteristic of Anti-sapstain Fungus Activity

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