Physiological Adaptation of Sorbus pohuashanensis Seedlings to Heat Stress

PENG Song; ZHENG Yong-qi; MA Miao; ZHANG Chuan-hong; DU Xiao-juan; LI Tao; NI Yan-sheng

Volume 24 Issue 5

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Physiological Adaptation of Sorbus pohuashanensis Seedlings to Heat Stress

1.
College of Life Science, Shihezi University, Shihezi 832000, Xinjiang, China
2.
Research Institute of Forestry, Chinese Academy of Forestry, Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091, China
3.
Xishan Forest Farm of Beijing Landscaping Services, Beijing 100093, China
4.
The Design Team of Wangqing Forestry Bureau, Wangqing 133200, Jilin, China

Received Date: 2010-06-22

Abstract

The chlorophyll fluorescence parameters, the contents of total chlorophyll and malondialdehyde (MDA), superoxide dismutase (SOD) activity, ascorbate peroxidase (APX) activity and chloroplast ultrastructure of one-year-old Sorbus pohuashanensis seedlings stressed by high temperature (40 ℃) in growth chamber were determined to elucidate its physiological response to heat stress. The results indicated that the contents of total chlorophyll decreased, electrolytic leakage and MDA increased,the activity of SOD and APX increased at first and then decreased with the increasing time of heat stress. The parameters of the largest quantum yield(Fv/Fm), actual photochemical efficiency(ΦPSⅡ), and photochemical quenching coefficient(qP)decreased except for an increase of non-photochemical quenching coefficient(NPQ). Chloroplast ultra-structure was serious damaged by 8 h heat stress, displaying collapsed chloroplast envelopes,blurred grana lamellae and disordered stroma lamellar. It is concluded that the seedlings of Sorbus pohuashanensis are sensitive to high temperature. It is proposed that the seedlings should be cultivated under partial shading at lower altitudes.
- high temperature stress,
- Sorbus pohuashanensis (Hance) Hedl,
- chlorophyll content,
- chlorophyll fluorescence,
- antioxidation system,
- chloroplast ultrastructure

References

[1]	傅立国, 陈潭清, 郎楷永,等. 中国高等植物[M]. 青岛:青岛出版社, 2003: 537-548
[2]	姜雁, 李近雨. 花楸育苗及引种试验初报[J]. 河北林业科技, 1998 (4): 1-4
[3]	姜雁, 杨靖宇. 花楸引种试验的阶段性总结[M]. 河北林业科技, 2001 (6): 17-19
[4]	陈建勋, 王晓峰. 植物生理学实验指导[J]. 广州:华南理工大学出版社,2002
[5]	李合生, 孙辉, 赵世杰. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2000
[6]	章家恩. 生态学常用实验研究方法与技术[M]. 北京: 化学工业出版社, 2007: 1-78
[7]	张振贤, 郭延奎, 艾希珍,等. 日光温室光温因子对黄瓜叶绿体超微结构及其功能的影响[J]. 应用生态学报, 2003, 14(8): 1287-1290
[8]	Asada K. Ascorbate peroxidase-a hydrogen peroxide scavenging enzyme in plants[J]. Physiol Plant, 1992, 85: 235-241
[9]	许大全, 张玉忠, 张荣铣. 植物光合作用的光抑制[J]. 植物生理学通讯, 1992, 28(4): 237-243
[10]	Kate M, Giles N J. Chlorophyll fluorescence-a practical guide[J]. Journal of Experimental Botany, 2000, 51(345): 659-668
[11]	Ruban A V, Horton P. Regulation of non-photochemical quenching of chlorophyll fluorescence in plants[J]. Australian Journal of plant Physiology, 1995, 22: 221-230
[12]	Ciamporova M, Mistrik I. The ultrastructural response of root cells to stressful conditions[J]. Environmental and Experimental Botany, 1993, 33: 11-26
[13]	Collins G G, Nie X L, Sahveit M E. Heat shock proteins and chilling sensitivity of mung bean hypocotyls[J]. Journal of Experimental Botany, 1995, 46: 795-802
[14]	Chia L S, McRae D G, Thompson J E. Light-dependence of paraquat-initiated membrane deterioration in bean plants. Evidence for the involvement of superoxide [J]. Physiol Plant, 1982, 56: 492-499
[15]	Lin C Y, Chen Y M, Key J L. Solute leakage in soybean seedlings under various heat shock regimes[J]. Plant Cell Physiology, 1985, 26(8): 1493-1498
[16]	苗琛, 利容千, 王建波. 甘蓝热胁迫叶片细胞的超微结构研究[J]. 植物学报, 1994, 36(9): 730-732
[17]	尹贤贵, 罗庆熙, 王文强. 番茄耐热性鉴定方法研究[J]. 西南农业学报, 2001, 14(2): 62-65
[18]	Ann C, Jaco V, Herman C. The redox status of plant cells (AsA and GSH) is sensitive to zinc imposed oxidative stress in roots and primary leaves of Phaseolus vulgaris[J]. Plant Physiology and Biochemistry, 2001, 39: 657-664
[19]	耶兴元, 马锋旺, 王顺才,等. 高温胁迫对猕猴桃幼苗叶片某些生理效应的影响[J]. 西北农林科技大学学报:自然科学版, 2004, 32(12): 33-37
[20]	汪炳良, 徐敏, 史庆华,等. 高温胁迫对早熟花椰菜叶片抗氧化系统和叶绿素及其荧光参数的影响[J]. 中国农业科学, 2004, 37(8): 1245-1250
[21]	汤日圣, 张大栋, 童红玉. 高温胁迫对稻苗某些生理指标的影响及ABA和6-BA对其的调节[J]. 江苏农业学报, 2005, 21(3): 145-149
[22]	曲复宁, 王云山, 张敏,等. 高温胁迫对仙客来根系活力和叶片生化指标的影响[J]. 华北农学报, 2002, 17(1): 127-131
[23]	Willekens H, Camp W V, Montagu M V,et al. Sulfur dioxide,and ultraviolet-B have similar effect on mRNA accumulation of antioxidant genes in Nicotiana plumbaginifolia L[J]. Plant Physiology, 1994, 106: 1007-1014
[24]	裘丽珍, 黄有军, 黄坚钦,等. 不同耐盐性植物在盐胁迫下的生长与生理特性比较研究[J]. 浙江大学学报:农业与生命科学版, 2006, 32(4): 420-427
[25]	Michel H, Florence T. Loss of chlorophyll with limited reduction of photosynthesis as an adaptive response of Syrian barley landraces to high-light and heat stress[J]. Australian Journal of Plant Physiology, 1999, 26: 569-578
[26]	陈立松, 刘星辉. 高温胁迫对桃和柚细胞膜透性和光合色素的影响[J]. 武汉植物学研究, 1997, 15(3): 233-237
[27]	谢寅峰, 黄晗, 汤玉香. 镧对汞胁迫下矢竹叶片生理反应的调节[J]. 林业科学, 2007, 43(12): 39-44
[28]	Santarius K A. Sites of heat sensitivity in chloroplasts and differential inactivation of cyclic and noncyclic photophosphorylation by heating[J]. Journal of Thermal Biology, 1975, 1: 101-107
[29]	Kocheva K, Lambrev p, Georgiev G,et al. Evaluation of chlorophyll fluorescence and membrane injury in the leaves of bareley cultivars under osmotic stress[J]. Bioelectrochemistry, 2004, 63: 121-124
[30]	王梅, 高志奎, 黄瑞虹,等. 茄子光系统Ⅱ的热胁迫特性[J]. 应用生态学报, 2007, 18(1): 63-68
[31]	Strasser R J, Tsimill-Michael M, Srivastava A. The fluorescence transient as a tool to characterise and screen photosynthetic samples [M]//. Yunus M, Pathre U and Mohanty P. Probing Photosynthesis: Mechanism, Regulation and Adaptation. London: Taylor and Francis, 2000
[32]	Unlai Tng, Xiaogang Wen, Qingtao Lu,et al. Heat Stress Induces an Aggregation of the Light-Harvesting Complex of PhotosystemⅡ in Spinach Plants[J]. Plant Physiology, 2007, 143: 629-638
[33]	李建建, 常雅君, 郁继华. 高温胁迫下黄瓜幼苗的某些光合特性和PSⅡ光化学活性的变化[J]. 植物生理学通讯, 2007, 43(6): 1085-1088
[34]	吴中伦. 国外树种引种概论[M]. 北京: 科学出版社, 1983
[35]	王明庥. 林木遗传育种[M]. 北京: 中国林业出版社, 2001
[36]	叶桂艳. 中国木兰科树种[M] 北京:中国农业出版社, 1996:75-78

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Physiological Adaptation of Sorbus pohuashanensis Seedlings to Heat Stress

1. College of Life Science, Shihezi University, Shihezi 832000, Xinjiang, China

2. Research Institute of Forestry, Chinese Academy of Forestry, Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091, China

3. Xishan Forest Farm of Beijing Landscaping Services, Beijing 100093, China

4. The Design Team of Wangqing Forestry Bureau, Wangqing 133200, Jilin, China

Received Date: 2010-06-22

Abstract: The chlorophyll fluorescence parameters, the contents of total chlorophyll and malondialdehyde (MDA), superoxide dismutase (SOD) activity, ascorbate peroxidase (APX) activity and chloroplast ultrastructure of one-year-old Sorbus pohuashanensis seedlings stressed by high temperature (40 ℃) in growth chamber were determined to elucidate its physiological response to heat stress. The results indicated that the contents of total chlorophyll decreased, electrolytic leakage and MDA increased,the activity of SOD and APX increased at first and then decreased with the increasing time of heat stress. The parameters of the largest quantum yield(Fv/Fm), actual photochemical efficiency(ΦPSⅡ), and photochemical quenching coefficient(qP)decreased except for an increase of non-photochemical quenching coefficient(NPQ). Chloroplast ultra-structure was serious damaged by 8 h heat stress, displaying collapsed chloroplast envelopes,blurred grana lamellae and disordered stroma lamellar. It is concluded that the seedlings of Sorbus pohuashanensis are sensitive to high temperature. It is proposed that the seedlings should be cultivated under partial shading at lower altitudes.

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Reference (36)

[1]	傅立国, 陈潭清, 郎楷永,等. 中国高等植物[M]. 青岛:青岛出版社, 2003: 537-548
[2]	姜雁, 李近雨. 花楸育苗及引种试验初报[J]. 河北林业科技, 1998 (4): 1-4
[3]	姜雁, 杨靖宇. 花楸引种试验的阶段性总结[M]. 河北林业科技, 2001 (6): 17-19
[4]	陈建勋, 王晓峰. 植物生理学实验指导[J]. 广州:华南理工大学出版社,2002
[5]	李合生, 孙辉, 赵世杰. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2000
[6]	章家恩. 生态学常用实验研究方法与技术[M]. 北京: 化学工业出版社, 2007: 1-78
[7]	张振贤, 郭延奎, 艾希珍,等. 日光温室光温因子对黄瓜叶绿体超微结构及其功能的影响[J]. 应用生态学报, 2003, 14(8): 1287-1290
[8]	Asada K. Ascorbate peroxidase-a hydrogen peroxide scavenging enzyme in plants[J]. Physiol Plant, 1992, 85: 235-241
[9]	许大全, 张玉忠, 张荣铣. 植物光合作用的光抑制[J]. 植物生理学通讯, 1992, 28(4): 237-243
[10]	Kate M, Giles N J. Chlorophyll fluorescence-a practical guide[J]. Journal of Experimental Botany, 2000, 51(345): 659-668
[11]	Ruban A V, Horton P. Regulation of non-photochemical quenching of chlorophyll fluorescence in plants[J]. Australian Journal of plant Physiology, 1995, 22: 221-230
[12]	Ciamporova M, Mistrik I. The ultrastructural response of root cells to stressful conditions[J]. Environmental and Experimental Botany, 1993, 33: 11-26
[13]	Collins G G, Nie X L, Sahveit M E. Heat shock proteins and chilling sensitivity of mung bean hypocotyls[J]. Journal of Experimental Botany, 1995, 46: 795-802
[14]	Chia L S, McRae D G, Thompson J E. Light-dependence of paraquat-initiated membrane deterioration in bean plants. Evidence for the involvement of superoxide [J]. Physiol Plant, 1982, 56: 492-499
[15]	Lin C Y, Chen Y M, Key J L. Solute leakage in soybean seedlings under various heat shock regimes[J]. Plant Cell Physiology, 1985, 26(8): 1493-1498
[16]	苗琛, 利容千, 王建波. 甘蓝热胁迫叶片细胞的超微结构研究[J]. 植物学报, 1994, 36(9): 730-732
[17]	尹贤贵, 罗庆熙, 王文强. 番茄耐热性鉴定方法研究[J]. 西南农业学报, 2001, 14(2): 62-65
[18]	Ann C, Jaco V, Herman C. The redox status of plant cells (AsA and GSH) is sensitive to zinc imposed oxidative stress in roots and primary leaves of Phaseolus vulgaris[J]. Plant Physiology and Biochemistry, 2001, 39: 657-664
[19]	耶兴元, 马锋旺, 王顺才,等. 高温胁迫对猕猴桃幼苗叶片某些生理效应的影响[J]. 西北农林科技大学学报:自然科学版, 2004, 32(12): 33-37
[20]	汪炳良, 徐敏, 史庆华,等. 高温胁迫对早熟花椰菜叶片抗氧化系统和叶绿素及其荧光参数的影响[J]. 中国农业科学, 2004, 37(8): 1245-1250
[21]	汤日圣, 张大栋, 童红玉. 高温胁迫对稻苗某些生理指标的影响及ABA和6-BA对其的调节[J]. 江苏农业学报, 2005, 21(3): 145-149
[22]	曲复宁, 王云山, 张敏,等. 高温胁迫对仙客来根系活力和叶片生化指标的影响[J]. 华北农学报, 2002, 17(1): 127-131
[23]	Willekens H, Camp W V, Montagu M V,et al. Sulfur dioxide,and ultraviolet-B have similar effect on mRNA accumulation of antioxidant genes in Nicotiana plumbaginifolia L[J]. Plant Physiology, 1994, 106: 1007-1014
[24]	裘丽珍, 黄有军, 黄坚钦,等. 不同耐盐性植物在盐胁迫下的生长与生理特性比较研究[J]. 浙江大学学报:农业与生命科学版, 2006, 32(4): 420-427
[25]	Michel H, Florence T. Loss of chlorophyll with limited reduction of photosynthesis as an adaptive response of Syrian barley landraces to high-light and heat stress[J]. Australian Journal of Plant Physiology, 1999, 26: 569-578
[26]	陈立松, 刘星辉. 高温胁迫对桃和柚细胞膜透性和光合色素的影响[J]. 武汉植物学研究, 1997, 15(3): 233-237
[27]	谢寅峰, 黄晗, 汤玉香. 镧对汞胁迫下矢竹叶片生理反应的调节[J]. 林业科学, 2007, 43(12): 39-44
[28]	Santarius K A. Sites of heat sensitivity in chloroplasts and differential inactivation of cyclic and noncyclic photophosphorylation by heating[J]. Journal of Thermal Biology, 1975, 1: 101-107
[29]	Kocheva K, Lambrev p, Georgiev G,et al. Evaluation of chlorophyll fluorescence and membrane injury in the leaves of bareley cultivars under osmotic stress[J]. Bioelectrochemistry, 2004, 63: 121-124
[30]	王梅, 高志奎, 黄瑞虹,等. 茄子光系统Ⅱ的热胁迫特性[J]. 应用生态学报, 2007, 18(1): 63-68
[31]	Strasser R J, Tsimill-Michael M, Srivastava A. The fluorescence transient as a tool to characterise and screen photosynthetic samples [M]//. Yunus M, Pathre U and Mohanty P. Probing Photosynthesis: Mechanism, Regulation and Adaptation. London: Taylor and Francis, 2000
[32]	Unlai Tng, Xiaogang Wen, Qingtao Lu,et al. Heat Stress Induces an Aggregation of the Light-Harvesting Complex of PhotosystemⅡ in Spinach Plants[J]. Plant Physiology, 2007, 143: 629-638
[33]	李建建, 常雅君, 郁继华. 高温胁迫下黄瓜幼苗的某些光合特性和PSⅡ光化学活性的变化[J]. 植物生理学通讯, 2007, 43(6): 1085-1088
[34]	吴中伦. 国外树种引种概论[M]. 北京: 科学出版社, 1983
[35]	王明庥. 林木遗传育种[M]. 北京: 中国林业出版社, 2001
[36]	叶桂艳. 中国木兰科树种[M] 北京:中国农业出版社, 1996:75-78

Physiological Adaptation of Sorbus pohuashanensis Seedlings to Heat Stress

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通讯作者: 陈斌, bchen63@163.com

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Physiological Adaptation of Sorbus pohuashanensis Seedlings to Heat Stress

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