Cloning and Expression Analysis of Chalcone Isomerase Gene cDNA from Camellia nitidissima

ZHOU Xing-wen; LI Ji-yuan; FAN Zheng-qi

A full-length cDNA sequence of chalcone isomerase (CHI) gene was obtained from petals of Camellia nitidissima using the methods of Reverse Transcription PCR and RACE,named Cn-CHI (GenBank accession No. HQ269805.1). Sequence analysis indicated that Cn-CHI is 953bp in full length and contains a 5'-untranslated region (5'-UTR) of 56 bp,a 3'-UTR of 204 bp,and an opening reading frame (ORF) of 693bp encoding a 230 predicted amino acids. Sequence alignment of amino acids revealed that Cn-CHI shared more than 75% homology with CHI from plants of Rosaceae, Ericaceae, Solanaceae and 99% homology with C. sinensis. Relative real-time PCR analysis indicated that Cn-CHI showed the tendency of a sharp increase at the early stage and then decreased slowly; the highest transcript abundance in carpels, and the second in stamens, the transcript of Cn-CHI in sepals and petals were almost at the same level.

1. Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang 311400, Zhejiang, China

2. Yulin Normal University, Yulin 537000, Guangxi, China

Received Date: 2011-06-28

Available Online: 2012-02-16

Abstract: A full-length cDNA sequence of chalcone isomerase (CHI) gene was obtained from petals of Camellia nitidissima using the methods of Reverse Transcription PCR and RACE,named Cn-CHI (GenBank accession No. HQ269805.1). Sequence analysis indicated that Cn-CHI is 953bp in full length and contains a 5'-untranslated region (5'-UTR) of 56 bp,a 3'-UTR of 204 bp,and an opening reading frame (ORF) of 693bp encoding a 230 predicted amino acids. Sequence alignment of amino acids revealed that Cn-CHI shared more than 75% homology with CHI from plants of Rosaceae, Ericaceae, Solanaceae and 99% homology with C. sinensis. Relative real-time PCR analysis indicated that Cn-CHI showed the tendency of a sharp increase at the early stage and then decreased slowly; the highest transcript abundance in carpels, and the second in stamens, the transcript of Cn-CHI in sepals and petals were almost at the same level.

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Reference (20)

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Cloning and Expression Analysis of Chalcone Isomerase Gene cDNA from Camellia nitidissima

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