Effect of Plant Hormones on Callus Induction and Regeneration of Stem Segment and Leaf from Acer negundo
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1.
College of Landscape Architecture, Northeast Forestry University, Harbin 150040, Heilongjiang, China
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2.
Shanghai Vocational and
Technical College of Agriculture and Forestry, Shanghai 201600, China
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3.
Research Institute of Forest Ecology, Environment
and Protection, Chinese Academy of Forestry, Beijing 100091, China
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Received Date:
2011-02-20
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Abstract
The stem segments and leaves of Acer negundo as explants were put on MS basis culture media with various hormones matching. The results indicated that the callus was induced from leaves on all kinds of culture media, but without the adventitious buds obtained. The callus induced from stem segments was found on several types of culture media, while the regeneration buds were only found on MS+0.000 5 mg·L-1 TDZ + 0.01 mg·L-1 NAA and differentiation ratio of buds was 22.8%. The rooting rate was 83.3% on MS + 0.1 mg·L-1 NAA. The survival rate of transplantion was 86.2%.
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