Analysis of the Gut Microbes of the Chinese White Wax Scale Ericerus pela and Molecular Detection of Rickettsia

XU Dong-li; LIU Wei-wei; HU Yan-hong; YANG Pu; CHEN Xiao-ming

To understand the diversity of the gut microbes of Chinese white wax scale Ericerus pela, 16S rDNA clone library was constructed to analyze the gut microbes of the female E. pela adults. The results showed that, the gut of E. pela contained abundant of Rickettsia. Gut microbes Arsenophonus and Variovorax were also been identified. And Rickettsia was the main bacteria in gut of E. pela. The phylogenetic analysis of the 16S rDNA showed that, the Rickettsia from E.pela has close relationship with that of pea aphid Acyrthosiphon pisum. PCR amplification was performed using the specific primers for Rickettsia to detect infection in 30 male and 30 female individuals. The result showed that, the infected male and female were 24 and 12 respectively.

1. Research Institute of Resource Insects, Chinese Academy of Forestry, Key laboratory of Cultivating and Utilization of Resources Insects of State Forestry Administration, Kunming 650224, Yunnan, China

Received Date: 2012-07-15

Available Online: 2012-12-13

Abstract: To understand the diversity of the gut microbes of Chinese white wax scale Ericerus pela, 16S rDNA clone library was constructed to analyze the gut microbes of the female E. pela adults. The results showed that, the gut of E. pela contained abundant of Rickettsia. Gut microbes Arsenophonus and Variovorax were also been identified. And Rickettsia was the main bacteria in gut of E. pela. The phylogenetic analysis of the 16S rDNA showed that, the Rickettsia from E.pela has close relationship with that of pea aphid Acyrthosiphon pisum. PCR amplification was performed using the specific primers for Rickettsia to detect infection in 30 male and 30 female individuals. The result showed that, the infected male and female were 24 and 12 respectively.

HTML

Reference (21)

[1]	Broderick N A, Raffa K F, Goodman R M, et al. Census of the bacterial community of the gypsy moth larval midgut by using culturing and culture-independent methods[J]. Applied and Environmental Microbiology, 2004,70(1): 293-300
[2]	相辉, 黄勇平. 肠道微生物与昆虫的共生关系[J]. 昆虫学报, 2008,45(5): 687-693
[3]	Sandstrom J, Moran N. How nutritionally imbalanced is phloem sap for aphids[J]. Entomologia Experimentalis et Applicata, 1999,91(1): 203-210
[4]	Douglas A. Phloem-sap feeding by animals: problems and solutions[J]. Journal of Experimental Botany, 2006,57(4): 747-754
[5]	Moran N A, Plague G R, Sandstrom J P, et al. A genomic perspective on nutrient provisioning by bacterial symbionts of insects[J]. Proceedings of the National Academy of Sciences of the United States of America, 2003,100(2): 14543-14548
[6]	陈晓鸣. 白蜡虫自然种群生态学[M]. 北京:科学出版社, 2011:1-25
[7]	Gottlieb Y, Ghanim M, Chiel E, et al. Identification and Localization of a Rickettsia sp. in Bemisia tabaci (Homoptera: Aleyrodidae)[J]. Applied and Environmental Microbiology, 2006,72(5): 3646-3652
[8]	Xiang H, Xie L, Zhang J, et al. Intracolonial differences in gut bacterial community between worker and soldier castes of Coptotermes formosanus[J]. Insect Science, 2011,19(1): 86-95
[9]	Mrázek J, Strosová L, Fliegerová K, et al. Diversity of insect intestinal microflora[J]. Folia Microbiologica, 2008,53(3): 229-233
[10]	刘莉, 王中康, 愈和韦, 等. 贡嘎蝠蛾幼虫肠道细菌多样性分析[J]. 微生物学报, 2008,48(5): 616-622
[11]	Behar A, Yuval B, Jurkevitch E. Gut bacterial communities in the Mediterranean fruit fly (Ceratitis capitata) and their impact on host longevity[J]. Journal of Insect Physiology, 2008,54(9): 1377-1383
[12]	Nishino S F, Spain J C. Biodegradation of 3-nitrotyrosine by Burkholderia sp. strain JS165 and Variovorax paradoxus JS171[J]. Applied and Environmental Microbiology, 2006,72(2): 1040-1044
[13]	Leadbetter J R, Greenberg E P. Metabolism of acyl-homoserine lactone quorum-sensing signals by Variovorax paradoxus[J]. Journal of Bacteriology, 2000,182(24): 6921-6926
[14]	Mouton L, Thierry M, Henri H, et al. Evidence of diversity and recombination in Arsenophonus symbionts of the Bemisia tabaci species complex[J]. Microbiology, 2012,12(Suppl 1): 10
[15]	Gherna R L, Werren J H, Weisburg W, et al. NOTES: Arsenophonus nasoniae gen. nov., sp. nov., the causative agent of the son-killer trait in the parasitic wasp Nasonia vitripennis[J]. International Journal of Systematic Evolutionary Microbiology, 1991,41(4):563-565
[16]	Skinner S W. Son-killer: a third extrachromosomal factor affecting sex ratio in the parasitoid wasp, Nasonia (=Mormoniella) vitripennis[J]. Genetics, 1985,109(4): 745-759
[17]	Hypsa V, Dale C. In vitro culture and phylogenetic analysis of "Candidatus Arsenophonus triatominarum," an intracellular bacterium from the triatomine bug, Triatoma infestans[J]. International Journal of Systematic and Evolutionary Microbiology, 1997,47(4):1140-1144
[18]	Dale C, Beeton M, Harbison C, et al. Isolation, pure culture, and characterization of "Candidatus Arsenophonus arthropodicus," an intracellular secondary endosymbiont from the hippoboscid louse fly Pseudolynchia canariensis[J]. Applied and Environmental Microbiology, 2006,72(4):2997-3004
[19]	刘魏魏, 杨璞, 阮永明, 等. 白蜡虫体内杀雄菌属 (Arsenophonus) 共生菌的分子检测[J]. 微生物学报, 2012,52(8):1002-1010
[20]	Perotti M A, Clarke H K, Turner B D, et al. Rickettsia as obligate and mycetomic bacteria[J]. The FASEB Journal, 2006,20(13):2372-2374
[21]	谭周进, 谢丙炎, 周清明, 等. 烟粉虱内共生菌16S rDNA的特性研究[J]. 微生物通报, 2007,34(3): 487-491

Analysis of the Gut Microbes of the Chinese White Wax Scale Ericerus pela and Molecular Detection of Rickettsia

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Proportional views