Isolation and Identification for Symbiotic Mycorrhizal Fungi of Rhododendron changii

LIU Zhen-hua; YAO Na; YANG Kai; WANG Tao; LI Zhen-jian; LI Lu-bin

Rhododendron changii is a monotypic species in China. This study isolated and obtained 10 same mycorrhizal fungi strains from Rhododendron changii and identified them by traditional classification method of morphology in conjunction with 18S rDNA sequence analysis. The results of identification suggest that this mycorrhizal fungi is Meliniomyces variabilis' sibling species.

1. Research Institute of Forestry, Chinese Academy of Forestry, Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091, China

2. Beijing Key Laboratory for Agriculgtural Application and New Technique, Beijing Agricultural College, Beijing 102206, China

Received Date: 2010-06-30

Available Online: 2012-12-13

Abstract: Rhododendron changii is a monotypic species in China. This study isolated and obtained 10 same mycorrhizal fungi strains from Rhododendron changii and identified them by traditional classification method of morphology in conjunction with 18S rDNA sequence analysis. The results of identification suggest that this mycorrhizal fungi is Meliniomyces variabilis' sibling species.

HTML

Reference (15)

[1]	陈有民. 园林树木学[M]. 北京:中国林业出版社,1990:619-620
[2]	Read D J. Pezizella ericae sp. nov. the perfect state of a typical mycorr-izal endophyte of ericaceae [J]. Transactions of the British Mycological Society, 1974, 63: 381-382
[3]	Mclean C, Lawrie A C. Patterns of root colonization in epacridaceous plants collected from different sites [J]. Annals of Botany, 1996,77:405-411
[4]	Monreal M, Berch S M, Berbee M. Molecular diversity of ericoid mycorrhizal fungi [J]. Canadian Journal of Botany, 1999, 77: 1580-1594
[5]	Sharples J M, Chambers S M, Meharg A A, et al. Genentic diversity of root-associated fungal endophytes from Calluna vulgaris at contrasting field sites [J]. New Phytologist, 2000, 148: 153-162
[6]	Tamara R, Allen T R, Millar T, et al. Culturing and direct DNA extraction find different from the same ericoid mycorrhizal roots [J]. New Phytologist, 2003, 160: 255-272
[7]	Pearson V, Read D J. The biology of mycorrhiza in the Ericaceae. I. The isolation of the endophyte and synthesis of mycorrhizas in aseptic cultures [J]. New Phytologist, 1973, 72:371-379
[8]	Hambleton S, Egger K N, Currah R S. The genus Oidiodendron: species delimitation and phylogenetic relationships based on nuclear ribosomal DNA analysis [J]. Mycologia, 1998(b), 90:854-869
[9]	Duclos J L, Fortin J A. Effect of glucose and active charcoal on in vitro synthesis of ericoid mycorrhiza with Vaccinium spp[J]. New Phytologist, 1983, 94:95-102
[10]	Perotto S, Actis-perino E, Perugini J, et al. Molecular diversity of fungi from ericoid mycorrhizal roots[J]. Molecular Ecology, 1996, 5:123-131
[11]	Rogers J E, Leblond J D, Moncreiff C A. Phylogenetic relationship of Alexandrium monilatum (Dinophyceae) to other Alexandrium species based on 18S ribosomal RNA gene sequences [J]. Harmful Algae, 2006(5): 275-280
[12]	He J, Xu Z, Hughes J. Analyses of soil fungal communities in adjacent natural forest and hoop pine plantation ecosystems of subtropical Australia using molecular approaches based on 18S rRNA genes[J]. FEMS Microbiology Letters, 2005, 247: 91-100
[13]	庄彩云,李潞滨,胡陶,等. 适用于rDNA ITS分析的兰属植物菌根真菌培养及DNA提取方法 [J].北京农学院学报,2007, 22(3):4-6
[14]	White T J, Bruns T, Lee S. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics[M]//Innins M A, Gelfand D H, Sninsky J J. In PCR Protocols. San Diego, Calif, Academic Press, 1990:315-322
[15]	Hambleton S,Sigler L. Meliniomyces, a new anamorph genus for root-associated fungi with phylogenetic affinities Rhizoscyphus ericae (≡Hymenoscyphus ericae), Leotiomycetes [J]. Studies in Mycology, 2005,53: 1-27

Isolation and Identification for Symbiotic Mycorrhizal Fungi of Rhododendron changii

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Proportional views