Genetic Diversity of Allozyme Markers of Liquidambar formosana Hance
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1.
State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China
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2.
Nanjing Forestry University, Nanjing 210037, Jiangsu, China
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3.
Center for Forest Tree Breeding, Hubei Provincial Forestry Bureau, Wuhan 430079, Hubei, China
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Received Date:
2011-04-24
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Abstract
The leaf samples from 16 Liquidambar formosana Hance populations were analyzed to study allozyme diversity using vertical slab polyacrylamide gel electrophoresis, and 6 loci of 6 enzyme systems were recorded. The results showed:(1) The genetic structures were different among populations. Allele frequencies ranged from 0-1.00, 4 rare genes and 3 unique genes were found in the assayed populations. At least 1 rare gene was found in 9 populations and 1 unique gene in 6 populations. (2) At species level, the mean number of alleles per locus was 3, the mean number of effective alleles per locus was 1.855 7, the percentage of polymorphic loci was 100%, and the observed heterozygosity was 0.582, the expected heterozygosity was 0.443, the mean value of Shannon index was 0.711 0. Overall, the proportion of heterozygotes within these populations was excessive. (3) According to the UPGMA cluster analysis based on Nei's genetic distance, the populations were classified into two groups, the Jian'ou population was clearly separated from all other populations by a large distance from other populations, and the rest 15 populations forming a big group, in which the populations of Fengdu and Huangshan were more distant from the other 13 populations, and genetic distances among the 13 populations were smaller from each other. The grouping of populations by cluster analysis was generally in consistence with the pattern of geographic distribution of the populations.
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References
|
[1]
|
郑万钧.中国树木志(2) [M].北京:中国林业出版社,1985:1888-1889
|
|
[2]
|
张锡象,陈建松. 保护乡土树种维护生态平衡[J].中国林业,2004(9):32
|
|
[3]
|
Wilson J E.The biological diversity crisis[J].BioScience,1985,35(11):700-706 |
|
[4]
|
王淑珍.孢子诱变菌株CJL990灵芝过氧化物酶和酯酶同工酶的研究[J].上海师范大学学报:自然科学版,2000,29(4):69-73
|
|
[5]
|
丁 毅,宋远淳.大麦酯酶同工酶酶谱的聚类分析与遗传研究[J].武汉大学学报:自然科学版,1995,41(6):729-734
|
|
[6]
|
孙立军,高吉寅,关建平.中国大麦酯酶同工酶的多样性及其地理分布研究[J].作物品种资源,1995,2(2):1-5
|
|
[7]
|
李小林.世界苹果属植物酯酶同工酶分析[J].西南农业大学学报,1997,19(2):105-111
|
|
[8]
|
葛 颂.遗传多样性及其检测方法[M].北京:科学出版社,1994:68-77
|
|
[9]
|
王中仁.植物等位酶分析[M].北京:科学出版社,1996:38-43
|
|
[10]
|
丁小飞.同工酶技术在鹅掌楸属树种分类中的应用研究[J].湖北林业科技,2009(2):5-8
|
|
[11]
|
Hamrick J L.Isozymes and the analysis of genetic structure in plant populations[M]//Soltis D E,Soltis P S.Isozymes in Plant Biology,London:Chapman and Hall,1990:87-105 |
|
[12]
|
郑 健,郑勇奇.花楸树天然群体的遗传多样性研究[J].生物多样性,2008,6(6):562-569
|
|
[13]
|
竺利波.紫荆遗传多样性研究[J].中国农学通报,2007,7(3):138-145
|
|
[14]
|
Honey M T,Parks C R. Genetic divergence in Liquidambar styraciflua, L. formosana, and L.acalycina (Hamamelidaceae)[J]. Systematic otany, 1994,19(2):308-316 |
|
[15]
|
Daniel L H, Andrew G C. Principles of population genetics[M]. Sinauer Associates:Inc.,2007 |
|
[16]
|
庞广昌,姜冬梅.群体遗传多样性和数据分析[J]. 林业科学, 1995,31(6):543-550
|
|
[17]
|
胡树贵,李 艳.44个百合品种同工酶遗传多样性研究[J].西部林业科学,2009,38(4):27-35
|
|
[18]
|
李文英,顾万春.蒙古栎天然群体表型多样性研究[J].林业科学,2005,41(1):49-56
|
|
[19]
|
付增娟,张川红.黑荆和银荆的繁殖扩散和入侵潜力[J].林业科学,2006,42(10):52-58
|
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