Analysis on Dominant Population of Fungus and Bacteria from Healthy and Canker Disease-Infected Populus×euroamericana Bark

LI Yong; PIAO Chun-gen; HE Wei; GUO Li-min; CHANG Ju-pu; XIE Shou-jiang; GUO Min-wei; LIN Le-min

To explore species changes of dominant population of fungi and bacteria from healthy and canker disease-infected Populus× euroamericana, the cultivable bacteria and fungi from healthy and diseased poplar bark in Puyang city were isolated. The results show that the dominant species of fungi from healthy and diseased poplar bark are Alternaria alternata and Fusarium solani respectively, and the strains number of F. solani from diseased poplar bark are more than 85% of all the fungal isolates. And the bacterial dominant species of diseased poplar plants bark is Lonsdalea quercina, and the strains number of which are more than 67% of the all bacterial isolates, while the preponderant species of three healthy poplar bark samples are different. F. solani and L. quercina became the preponderant species in cankers of Populus×euroamericana.

1. Beijing Forestry University, Beijing 100083, China

2. Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing 100091, China

3. Puyang Forestry Institute, Puyang 457000, He'nan, China

4. Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China

Received Date: 2012-03-01

Abstract: To explore species changes of dominant population of fungi and bacteria from healthy and canker disease-infected Populus× euroamericana, the cultivable bacteria and fungi from healthy and diseased poplar bark in Puyang city were isolated. The results show that the dominant species of fungi from healthy and diseased poplar bark are Alternaria alternata and Fusarium solani respectively, and the strains number of F. solani from diseased poplar bark are more than 85% of all the fungal isolates. And the bacterial dominant species of diseased poplar plants bark is Lonsdalea quercina, and the strains number of which are more than 67% of the all bacterial isolates, while the preponderant species of three healthy poplar bark samples are different. F. solani and L. quercina became the preponderant species in cankers of Populus×euroamericana.

HTML

Reference (16)

[1]	贺伟,任飞娟,郭利民,等. 欧美杨溃疡病的病原鉴定[J]. 林业科学,2009,45(6):104-109
[2]	牟新涛,李永,李强军,等. 三峡库区云阳县三种类型马尾松林微生物区系分析 I. 林地土壤细菌、芽孢杆菌和真菌[J]. 林业科学研究,2010,23(4):560-566
[3]	牟新涛,李永,李强军,等. 三峡库区云阳县三种类型马尾松林微生物区系分析 II. 林地空气、叶面和树皮表面微生物区系. [J]. 林业科学研究,2010,23(5):762-769
[4]	陈华红,杨颖,姜怡,等. 植物内生放线菌的分离方法[J]. 微生物学通报,2006,33(4):182-185
[5]	王彦芹,席琳乔. 棉花根际促生菌基因组DNA 的提取及其鉴定[J]. 基因组学与应用生物学,2010,29(5)1005-1008
[6]	林彩丽,王曦茁,朴春根,等. 一种用于PCR扩增的真菌DNA快速提取方法. 专利号:896003
[7]	Galkiewicz J P, Kellogg C A. Cross-Kingdom Amplification, Using Bacteria-Specific Primers:Complications for Studies of Coral Microbial Ecology[J]. Applied and Environmental Microbiology, 2008, 74(2):7828-7831
[8]	White T J, Bruns T, Lee S, et al. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics[M]//Innis MA,Gelfand DH,Sninsky J J, et al. PCR Protocols:A Guide to Methods andApplications. New York:Academic Press, 1990:315- 322
[9]	Chun J, Lee J H, Jung Y, et al. EzTaxon:a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences[J]. Int J Syst Evol Microbiol, 2007,57:2259-2261
[10]	魏景超. 真菌鉴定手册[M]. 上海:上海科学技术出版社,1979
[11]	Xing X K, Chen J, Xu M J, et al. Fungal endophytes associated with Sonneratia (Sonneratiaceae) mangrove plants on the south coast of China[J]. For Path, 2011,41:334-340
[12]	Poza-Carrión C, Aguilar I, Gallego, et al. Brenneria quercina and Serratia spp. isolated from Spanish oak trees:molecular characterization and development of PCR primers[J]. Plant Pathology, 2008,57:308-319
[13]	Biosca E G, González R, López-López M J, et al. Isolation and Characterization of Brenneria quercina, Causal Agent for Bark Canker and Drippy Nut of Quercus spp. in Spain[J]. Phytopathology, 2003,93(4):485-492
[14]	Brady C L, Cleenwerck I, Denman S, et al. Proposal to reclassify Brenneria quercina (Hildebrand & Schroth 1967) Hauben et al. 1999 into a novel genus, Lonsdalea gen. nov., as Lonsdalea quercina comb. nov., descriptions of Lonsdalea quercina subsp. quercina comb. nov., Lonsdalea quercina subsp. iberica subsp. nov. and Lonsdalea quercina subsp. britannica subsp. nov., emendation of the description of the genus Brenneria, reclassification of Dickeya dieffenbachiae as Dickeya dadantii subsp. dieffenbachiae comb. nov., and emendation of the description of Dickeya dadantii[J]. Int J Syst Evol Microbiol,2012,62:1592-1602
[15]	Brennan N M, Ward A C, Beresford T P, et al. Biodiversity of the bacterial flora on the surface of a smear cheese[J].Appl Environ Microbiol, 2002,68(2):820-830
[16]	Schröder J, Maus I, Trost E, et al. Complete genome sequence of Corynebacterium variabile DSM 44702 isolated from the surface of smear-ripened cheeses and insights into cheese ripening and flavor generation[J]. BMC Genomics, 2011, 12:545

Analysis on Dominant Population of Fungus and Bacteria from Healthy and Canker Disease-Infected Populus×euroamericana Bark

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Proportional views