Research on Genetic Variations of Anthocyanins in Azalea Petals
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1.
School of Marine Sciences, Ningbo University, Ningbo 315211, Zhejiang, China
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2.
Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang 311400, Zhejiang, China
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3.
Ningbo City College of Vocational Technology, Ningbo 315502, Zhejiang, China
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Received Date:
2012-04-26
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Abstract
27 azalea samples of seven pairs of azalea parents and their hybrids were analyzed by High Performance Liquid Chromatography (HPLC) with UV/Vis Detector, and 15 major anthocyanins were detected. The results of quantitative analysis show that Cy3Ga, Cy3G, pigment 2, pigment 5 and pigment 14 are the main anthocyanins in azalea petals, with accumulated content over 90% of total anthocyanins, and Dp3G was only detected in 4 samples. Inheritance analysis indicates that Cy3G5G and pigment 15 are multiple-gene inheritance pattern dominated by enhancer genes; pigment 5, pigment 14 and Cy3G are controlled by equivalent genes; pigment 2, pigment 4 and Cy3G are dominated by reductive genes. Therefore it is deduced that flavonoid 3-O-glucosyltransferase and flavonoid 3-O-galactosyltransferase are controlled respectively by reductive genes and equivalent genes; flavonoid 5-O-galactosyltransferase is controlled by enhancer genes. The results of color comparison show that Dp3G is highly correlated with blue-purple in azalea flowers; pigment 14 is correlated with magenta flowers, and other anthocyanins are correlated with scarlet flowers. The strategies for color breeding with anthocyanin assistance are brought forward.
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