Detection of the DNA Methylation Difference Between the Triploid and Diploid Poplar Trees

CHEN Cheng-bin; XUE Zhen-yi; HU Bao-quan; WANG Chun-guo; SONG Wen-qin

The methylation-sensitive amplification polymorphism (MSAP) was used to analyze the methylation in triploid and diploid poplar trees. 745 loci were detected by gel electrophoresis. The total methylation ratios in different ploidy of poplar trees varied from 13.69% to 18.84%. And the average total methylation ratios were 14.71% for diploid and 17.72% for triploid trees. The total methylation rate of poplar populations increased with the ploidy, the semi-methylation in black poplar group also increased with the ploidy, while the opposite result was found in white poplar group. No regular pattern was found in the variation of total methylation ratios of black poplar group, and the total methylation rate of white poplar group increased with the ploidy. There exists a correlation between total methylation ratios and polyploidy level in poplar ploidy group (r=0.835, Pr=0.651, P<0.05). But no significant correlation between hemimethylation ratios and polyploidy level was observed.

1. College of Life Sciences, Nankai University, Tianjin 300071, China

Received Date: 2013-08-23

Abstract: The methylation-sensitive amplification polymorphism (MSAP) was used to analyze the methylation in triploid and diploid poplar trees. 745 loci were detected by gel electrophoresis. The total methylation ratios in different ploidy of poplar trees varied from 13.69% to 18.84%. And the average total methylation ratios were 14.71% for diploid and 17.72% for triploid trees. The total methylation rate of poplar populations increased with the ploidy, the semi-methylation in black poplar group also increased with the ploidy, while the opposite result was found in white poplar group. No regular pattern was found in the variation of total methylation ratios of black poplar group, and the total methylation rate of white poplar group increased with the ploidy. There exists a correlation between total methylation ratios and polyploidy level in poplar ploidy group (r=0.835, Pr=0.651, P<0.05). But no significant correlation between hemimethylation ratios and polyploidy level was observed.

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Detection of the DNA Methylation Difference Between the Triploid and Diploid Poplar Trees

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