Genetic Variation of Jinzaoshi Persimmon from Different Regions in Zhejiang Province

XIONG Chun-yan; GONG Bang-chu; YING Shang-jiao; WU Kai-yun; JIANG Xi-bing; ZHAO Xian-min

Volume 27 Issue 2

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Genetic Variation of Jinzaoshi Persimmon from Different Regions in Zhejiang Province

1.
Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang 311400, Zhejiang, China
2.
Forestry Bureau of Yongkang City, Zhejiang Province, Yongkang 321300, Zhejiang, China
3.
Forestry and Fruit Technical Service Station of Agricultural Economics Bureau of Haining City, Zhejiang Province, Haining 314400, Zhejiang, China

Received Date: 2013-05-23

Abstract

The germplasm resources of Jinzaoshi persimmon in Zhejiang Province were investigated, so as to probe the genetic diversity of Jinzaoshi persimmon from different regions. The genetic variation of collected germplams, including 37 Jinzaoshi and 3 local varieties, was analyzed by SSR molecular marker, together with phenotypic traits and fruit nutrients contents. The results showed Jinzaoshi persimmon possessed a rich diversity on morphology. The variable coefficient about 14 phenotypic characters ranged from 7.23% to 23.54%. The variable coefficient of sepal length and persimmon calyx length were the largest. The fruit shape index and leaf index had the least variation. The fruit nutrient contents ranged from 9.58% (soluble solid content) to 66.21% (tannin content). Twenty-one primers were used to amplify 381 SSR local in 40 test materials, with an average polymorphism rate of 92.89%. The cluster analysis conducted by UPGMA showed that the materials tested could be distinguished and divided into three major groups at the genetic similarity coefficient of 0.74, which was similar to the relationship of PCoA analysis.
- Jinzaoshi persimmon,
- phenotypic character,
- SSR,
- genetic diversity

References

[1]	吴连海,徐象华,颜福花,等.金枣柿繁育研究初报[J].湖南农业科学,2011,50(10):2019-2021
[2]	何小勇,何林,谢建秋.金枣柿的开发利用[J].中国林副特产,2006(2):47
[3]	郭大龙.几种分子标记技术的建立及其在部分柿属植物亲缘关系分析中的应用[D].武汉:华中农业大学,2006
[4]	Hu D C, Luo Z R. Polymorphisms of amplied mitochondrial DNA non-coding regions in Diospyros spp.[J].Scientia Horticulturae, 2006, 109(3): 275-281
[5]	Du X Y, Zhang Q L, Luo Z R. Comparison of four molecular markers for genetic analysis in Diospyros L.(Ebenaceae)[J].Plant Systematics and Evolution, 2009, 281(1-4): 171-181
[6]	胡燕.金枣柿的分类地位研究[D].武汉:华中农业大学,2009
[7]	杨勇,王仁梓,李高潮,等.柿属植物及柿品种染色体数目研究[J].西北农业学报,1999,8(3):64-67
[8]	赵献民,龚榜初,吴开云,等.浙江省农家柿品种数量分类研究[J].林业科学研究,2012,25(1):77-87
[9]	李根有,陈征海,裘宝林.浙江柿属一新种[J].浙江林学院学报,2006,2(34):378-381
[10]	徐象华,陈民管,李永强,等.金枣柿与部分柿属植物亲缘关系的ISSR分析[J].浙江农业学报,2010,22(6):731-735
[11]	杨勇,王仁梓,李高潮,等.柿种质资源描述规范和数据标准[M].北京:中国农业出版社,2006
[12]	国家林业局. 中华人民共和国林业行业标准 LY/T 1870-2010 植物新品种特异性、一致性、稳定性测试指南:柿[S]. 北京: 中国标准出版社,2010
[13]	赵献民.浙江省农家柿品种资源多样性及分类研究[D].北京:中国林业科学研究院,2010
[14]	Doyle J J, Doyle J L. A rapid DNA isolation procedure for small quantities of fresh leaf tissue[J]. Phytochemistry Bulletin, 1987, 19: 11-15
[15]	阮小凤,Krczal G,杨勇.磁珠富集法分离柿微卫星标记[J].西北农林科技大学学报:自然科学版,2008,36(5):97-102
[16]	Soriano J M, Pecchioli S, Romero C, et al. Deve1opment of microsatellite markers in po1yploid persimmon (Diospyros kaki L. f) from an enriched genomic library[J]. Molecular Ecology Notes, 2006, 6(2): 368-370
[17]	颜福花.李属植物及其近缘种的等位酶遗传多样性和种间关系研究[D].武汉:华中农业大学,2006
[18]	邓立宝,何新华,李天文,等.利用表型结合目标起始密码子多态性分子标记调查广西云贵高原地区柿种质资源状况[J].园艺学报,2012,39(2):215-224
[19]	何照范,牛爱珍,向显衡,等.刺梨果实营养及其维生素C含量变化的研究[J].园艺学报,1984,11(4):271-273
[20]	李树另,黄礼森,丛佩华,等.不同种内梨品种果实维生素C含量[J].园艺学报,1994,21(1):17-20
[21]	蔡永强,向青云,陈家龙,等.火龙果的营养成分分析[J].经济林研究,2008,26(4):53-56
[22]	Botta R, Scott N S, Eynard I, et al. Evaluation of microsatellite sequence-tagged site markers for characterizing Vitis vinifera cultivars[J]. Vitis, 1995, 34(2): 99-102
[23]	巴乔瑞,赵政阳,高华,等.基于SSR和SRAP标记的苹果品种亲缘关系分析[J].西北农林科技大学学报:自然科学版,2011,39(9):123-128
[24]	张东,舒群,滕元文,等.中国红皮沙梨品种的SSR标记分析[J].园艺学报,2007,34(1):47-52
[25]	张艳芳.利用形态学和SSR标记建立柿核心种质[D].武汉:华中农业大学,2010

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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Genetic Variation of Jinzaoshi Persimmon from Different Regions in Zhejiang Province

1. Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang 311400, Zhejiang, China

2. Forestry Bureau of Yongkang City, Zhejiang Province, Yongkang 321300, Zhejiang, China

3. Forestry and Fruit Technical Service Station of Agricultural Economics Bureau of Haining City, Zhejiang Province, Haining 314400, Zhejiang, China

Received Date: 2013-05-23

Abstract: The germplasm resources of Jinzaoshi persimmon in Zhejiang Province were investigated, so as to probe the genetic diversity of Jinzaoshi persimmon from different regions. The genetic variation of collected germplams, including 37 Jinzaoshi and 3 local varieties, was analyzed by SSR molecular marker, together with phenotypic traits and fruit nutrients contents. The results showed Jinzaoshi persimmon possessed a rich diversity on morphology. The variable coefficient about 14 phenotypic characters ranged from 7.23% to 23.54%. The variable coefficient of sepal length and persimmon calyx length were the largest. The fruit shape index and leaf index had the least variation. The fruit nutrient contents ranged from 9.58% (soluble solid content) to 66.21% (tannin content). Twenty-one primers were used to amplify 381 SSR local in 40 test materials, with an average polymorphism rate of 92.89%. The cluster analysis conducted by UPGMA showed that the materials tested could be distinguished and divided into three major groups at the genetic similarity coefficient of 0.74, which was similar to the relationship of PCoA analysis.

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Reference (25)

[1]	吴连海,徐象华,颜福花,等.金枣柿繁育研究初报[J].湖南农业科学,2011,50(10):2019-2021
[2]	何小勇,何林,谢建秋.金枣柿的开发利用[J].中国林副特产,2006(2):47
[3]	郭大龙.几种分子标记技术的建立及其在部分柿属植物亲缘关系分析中的应用[D].武汉:华中农业大学,2006
[4]	Hu D C, Luo Z R. Polymorphisms of amplied mitochondrial DNA non-coding regions in Diospyros spp.[J].Scientia Horticulturae, 2006, 109(3): 275-281
[5]	Du X Y, Zhang Q L, Luo Z R. Comparison of four molecular markers for genetic analysis in Diospyros L.(Ebenaceae)[J].Plant Systematics and Evolution, 2009, 281(1-4): 171-181
[6]	胡燕.金枣柿的分类地位研究[D].武汉:华中农业大学,2009
[7]	杨勇,王仁梓,李高潮,等.柿属植物及柿品种染色体数目研究[J].西北农业学报,1999,8(3):64-67
[8]	赵献民,龚榜初,吴开云,等.浙江省农家柿品种数量分类研究[J].林业科学研究,2012,25(1):77-87
[9]	李根有,陈征海,裘宝林.浙江柿属一新种[J].浙江林学院学报,2006,2(34):378-381
[10]	徐象华,陈民管,李永强,等.金枣柿与部分柿属植物亲缘关系的ISSR分析[J].浙江农业学报,2010,22(6):731-735
[11]	杨勇,王仁梓,李高潮,等.柿种质资源描述规范和数据标准[M].北京:中国农业出版社,2006
[12]	国家林业局. 中华人民共和国林业行业标准 LY/T 1870-2010 植物新品种特异性、一致性、稳定性测试指南:柿[S]. 北京: 中国标准出版社,2010
[13]	赵献民.浙江省农家柿品种资源多样性及分类研究[D].北京:中国林业科学研究院,2010
[14]	Doyle J J, Doyle J L. A rapid DNA isolation procedure for small quantities of fresh leaf tissue[J]. Phytochemistry Bulletin, 1987, 19: 11-15
[15]	阮小凤,Krczal G,杨勇.磁珠富集法分离柿微卫星标记[J].西北农林科技大学学报:自然科学版,2008,36(5):97-102
[16]	Soriano J M, Pecchioli S, Romero C, et al. Deve1opment of microsatellite markers in po1yploid persimmon (Diospyros kaki L. f) from an enriched genomic library[J]. Molecular Ecology Notes, 2006, 6(2): 368-370
[17]	颜福花.李属植物及其近缘种的等位酶遗传多样性和种间关系研究[D].武汉:华中农业大学,2006
[18]	邓立宝,何新华,李天文,等.利用表型结合目标起始密码子多态性分子标记调查广西云贵高原地区柿种质资源状况[J].园艺学报,2012,39(2):215-224
[19]	何照范,牛爱珍,向显衡,等.刺梨果实营养及其维生素C含量变化的研究[J].园艺学报,1984,11(4):271-273
[20]	李树另,黄礼森,丛佩华,等.不同种内梨品种果实维生素C含量[J].园艺学报,1994,21(1):17-20
[21]	蔡永强,向青云,陈家龙,等.火龙果的营养成分分析[J].经济林研究,2008,26(4):53-56
[22]	Botta R, Scott N S, Eynard I, et al. Evaluation of microsatellite sequence-tagged site markers for characterizing Vitis vinifera cultivars[J]. Vitis, 1995, 34(2): 99-102
[23]	巴乔瑞,赵政阳,高华,等.基于SSR和SRAP标记的苹果品种亲缘关系分析[J].西北农林科技大学学报:自然科学版,2011,39(9):123-128
[24]	张东,舒群,滕元文,等.中国红皮沙梨品种的SSR标记分析[J].园艺学报,2007,34(1):47-52
[25]	张艳芳.利用形态学和SSR标记建立柿核心种质[D].武汉:华中农业大学,2010

Genetic Variation of Jinzaoshi Persimmon from Different Regions in Zhejiang Province

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通讯作者: 陈斌, bchen63@163.com

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Genetic Variation of Jinzaoshi Persimmon from Different Regions in Zhejiang Province

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