Effects of Glucose Addition on Soil Nitrogen Transformation and Net Mineralization and Nitrification in Chinese Fir Plantation

MA Hong-liang; WEI Chun-lan; LI Lei; GAO Ren; YIN Yun-feng; YANG Yu-sheng

Volume 27 Issue 3

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Article Navigation > Forest Research > 2014 > 27(3): 356-362

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Effects of Glucose Addition on Soil Nitrogen Transformation and Net Mineralization and Nitrification in Chinese Fir Plantation

1.
Key Laboratory of Humid Subtropical Eco-geographical Process of Ministry of Education, Fuzhou 350007, Fujian, China
2.
College of Geographical Sciences, Fujian Normal University, Fuzhou 350007, Fujian, China

Received Date: 2013-04-28

Abstract

In view of the significant role of litter played in regulating forest soil nitrogen transformation, simulating the influence of matrix carbon sources on nitrogen transform in the subtropical coniferous forest acid soil is helpful to deeply understand the mechanism between carbon and nitrogen. The effects of glucose addition (as C of 0, 100, 300, 1 000, 2 000, and 5 000 mg·kg-1) on soil nitrogen content, nitrogen mineralization and nitrification were studied in the soil under subtropical Chinese fir plantation. The results showed that the glucose addition reduced the soil inorganic nitrogen content and proportion in total soluble nitrogen, and the nitrate nitrogen decreased more than ammonium nitrogen; but the decrease of soluble organic nitrogen (SON) and pH was not found, even the ratio of SON in total soluble nitrogen increased. Glucose addition decreased the net nitrogen mineralization and the nitrification rates for nitrogen mineralization were inhibited. The results showed that hydrophilic nitrogen proportion decreased significantly with the addition of glucose, which was related to nitrogen immobilization and transformation to result in the enhancement of SON proportion. Correlation analysis showed that there was a significantly negative linear relationship (R2 = 0.902) between the proportions of nitrate and dissolved organic nitrogen in the total nitrogen of the extract from soil, which indirectly give a evidence of glucose likely promoting the nitrate retaining and transformation by microorganisms involved in soil. The research discovered that the glucose amount (as C) of 1 000 mg ·kg-1 was the threshold affecting the nitrogen transforms in forest soil under subtropical Chinese fir plantation.
- nitrogen deposition,
- soil soluble organic carbon,
- forest litter,
- Chinese fir plantation,
- carbon source

References

[1]	Kaiser J. The other global pollutant: nitrogen proves tough to curb [J]. Science, 2001, 294: 268-269
[2]	Bobbink R, Hicks K, Galloway J, et al. Global assessment of nitrogen deposition effects on terrestrial plant diversity: a synthesis [J]. Ecological Applications, 2010, 20: 30-59
[3]	李德军, 莫江明, 方运霆, 等. 模拟氮沉降对南亚热带两种乔木幼苗生物量及其分配的影响[J]. 植物生态学报, 2005, 29 (4):543-549
[4]	郝吉明, 谢绍东, 段雷, 等. 酸沉降临界负荷及应用[M].北京:清华大学出版社, 2001
[5]	Chen X Y,Mulder J. Atmospheric deposition of nitrogen at five subtropical forested sites in South China [J]. Science of the Total Environment, 2007, 378: 317-330
[6]	张乐, 何红波, 章建新, 等. 不同用量葡萄糖对土壤氮素转化的影响[J]. 土壤通报, 2008, 39(4): 775-778
[7]	朱兆良. 农田中氮肥的损失与对策[J]. 土壤与环境, 2000, 9(1): 1-6
[8]	贾俊仙, 李忠佩, 车玉萍. 添加葡萄糖对不同肥力红壤性水稻土氮素转化的影响[J]. 中国农业科学, 2010, 43(8):1617-1624
[9]	贾俊仙, 李忠佩, 车玉萍. 添加葡萄糖对不同肥力黑土氮素转化的影响[J]. 土壤学报, 2011, 48(1): 207-211
[10]	Joergensen R G, Scheu S. Response of soil microorganisms to the addition of carbon, nitrogen and phosphorus in a forest Rendzina[J]. Soil Biology and Biochemistry, 1999, 31(6): 859-866
[11]	Joergensen R G, Raubuch M. Adenylate energy charge of a glucose-treated soil without adding a nitrogen source[J]. Soil Biology and Biochemistry, 2002, 34: 1317-1324
[12]	Yoshitake S, Sasaki A, Uchida M, et al. Carbon and nitrogen limitation to microbial respiration and biomass in an acidic solfatara field[J]. European Journal of Soil Biology, 2007, 43:1-13
[13]	Dendooven L, Alcántara-Hernández R J, Valenzuela-Encinas C, et al. Dynamics of carbon and nitrogen in an extreme alkaline saline soil: A review[J]. Soil Biology and Biochemistry, 2010, 42: 865-877
[14]	Venterea R T, Groffman P M, Verchot L V, et al. Gross nitrogen process rates in temperate forest soils exhibiting symptoms of nitrogen saturation[J]. Forest Ecology and Management, 2004, 196: 129-142
[15]	Szili-Kovács T, Török K, Tilston E L, et al. Promoting microbial immobilization of soil nitrogen during restoration of abandoned agricultural fields by organic additions[J]. Biology of Fertility and Soils, 2007, 43: 823-828
[16]	Chen X Y, Mulder J, Wang Y H, et al. Atmospheric deposition, mineralization and leaching of nitrogen in subtropical forested catchments, South China [J]. Environmental Geochemistry and Health, 2004, 26:179-186
[17]	Bouwman A F, Van Drecht G, Van der Hoek K W. Global and regional surface nitrogen balances in intensive agricultural production systems for the period 1970-2030 [J]. Pedosphere, 2005,15:137-155
[18]	Kanerva S, Smolander A. Microbial activities in forest floor layers under silver birch, Norway spruce and Scots pine [J]. Soil Biology and Biochemistry, 2007,39:1459-1467
[19]	Tan X, Chang S X. Soil compaction and forest litter amendment affect carbon and net nitrogen mineralization in a boreal forest soil [J]. Soil and Tillage Research, 2007, 93:77-86
[20]	Huang Z Q, Xu Z H, Blumfield T J, et al. Soil nitrogen mineralization and fate of (¹⁵NH₄)₂SO₄ in field incubated soil in a hardwood plantation of subtropical Australia: the effect of mulching [J]. Journal of Soils and Sediments, 2008, 8:389-397
[21]	Holub S M, Lajtha K, Spears J D H, et al. Organic matter manipulations have little effect on gross and net nitrogen transformations in two temperate forest mineral soils in the USA and central Europe [J]. Forest Ecology and Management, 2005, 214: 320-330
[22]	Xu X L, Ouyang H, Cao G M. Nitrogen retention patterns and their controlling factors in an alpine meadow: implications for carbon sequestration [J]. Biogeosciences Discuss, 2007,4: 2641-2665
[23]	Ste-Marie C, Houle D. Forest floor gross and net nitrogen mineralization in three forest types in Quebec, Canada [J]. Soil Biology and Biochemistry, 2006, 38: 2135-2143
[24]	向升华, 高人, 马红亮, 等. 不同年龄杉木人工林土壤无机氮比较研究[J]. 亚热带资源与环境学报, 2008, 3(1): 24-30
[25]	Zhang W, Mo J, Yu G, et al. Emissions of nitrous oxide from three tropical forests in Southern China in response to simulated nitrogen deposition [J]. Plant and Soil, 2008, 306:221-236
[26]	Zhao W, Cai Z, Xu Z. Does ammonium-based N addition influence nitrification and acidification in humid subtropical soils of China [J]. Plant Soil, 2007, 297:213-221
[27]	DeForest J L, Zak D R, Pregitzer K S, et al. Atmospheric nitrate deposition, microbial community composition, and enzyme activity in northern hardwood forests [J]. Soil Science Society of America Journal, 2004, 68:132-138
[28]	方运霆, 莫江明, Gundersen P, 等. 森林土壤氮素转换及其对氮沉降的响应[J]. 生态学报, 2004, 24(7): 1523-1531
[29]	邓小文, 韩士杰. 氮沉降对森林生态系统土壤碳库的影响[J].生态学杂志, 2007, 26(10): 1622-1627
[30]	Vesterdal L, Schmidt I K., Callesen I, et al. Carbon and nitrogen in forest floor and mineral soil under six common European tree species[J]. Forest Ecology and Management, 2008, 255: 35-48
[31]	罗献宝,张颖清,徐浩,等. 温带阔叶红松林中不同树种和倒木对土壤性质的影响[J]. 生态环境学报, 2011, 20(12): 1841-1845
[32]	Gilliam F S, Lyttle N L, Thomas A, et al. Soil variability along a nitrogen mineralization and nitrification gradient in a nitrogen-saturated hardwood forest[J]. Soil Science Society of American Journal, 2005,69:247-256
[33]	Magill A H, Aber J D. Variation in soil net mineralization rates with dissolved organic carbon additions[J]. Soil Biology and Biochemistry, 2000, 32:597-601
[34]	Zhang J B, Cai Z C, Zhu T B. N₂O production pathways in the subtropical acid forest soils in China[J]. Environmental Research, 2011,111: 643-649
[35]	钱琛. 亚热带红壤的硝化作用及其对NO₃-N淋溶和土水酸化的影响[D]. 南京: 中国科学院南京土壤研究所, 2008
[36]	俞慎, 李振高. 稻田生态系统生物硝化-反硝化作用与氮素损失[J]. 应用生态学报, 1999, 10 (5) : 630-634
[37]	Lu S, Hu H, Sun Y, et al. Effect of carbon source on the denitrification in constructed wetlands[J]. Journal of Environmental Sciences,2009,21: 1036-1043
[38]	Jordan T E, Weller D E, Correll D L. Denitrification in surface soils of a riparian forest: Effects of water, nitrate and sucrose additions[J]. Soil Biology and Biochemistry, 1998, 30:833-843
[39]	葛晓改, 肖文发, 曾立雄, 等. 不同林龄马尾松凋落物基质质量与土壤养分的关系[J]. 生态学报, 2012, 32(3) : 852-862
[40]	刘维丽, 马红亮, 彭秀明, 等. 凋落物中次生代谢物对森林土壤可溶性氮的影响[J]. 土壤, 2010,42(4):564-568
[41]	王杰, 马红亮, 高人, 等. 添加酚类物质对森林土壤无机氮的影响[J].安徽农业科学, 2010, 38(10): 5184-5187
[42]	马红亮, 刘维丽, 高人, 等. 凋落物与单宁酸对森林土壤无机氮的影响[J].应用生态学报, 2011, 22(1): 61-65
[43]	Dodla S K, Wang J J, DeLaune R D, et al. Denitrification potential and its relation to organic carbon quality in three coastal wetland soils[J]. Science of the Total Environment, 2008, 407: 471-480
[44]	Ma H L, Gao R, Wang J, et al. Response of soil NH₄⁺ and NO₃^- transformations to nitrogen deposition under addition of litters and tannin [M]// Li S C, Wang W Y, Niu P H, et al. Progress in Environmental Science and Technology (VOL.Ⅲ) .Beijing Jiaotong University, Science Press, 2011, 54-59
[45]	Lummer D, Scheu S, Butenschoen O. Connecting litter quality, microbial community and nitrogen transfer mechanisms in decomposing litter mixtures [J]. Oikos, 2012, 121: 1649-1655

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Effects of Glucose Addition on Soil Nitrogen Transformation and Net Mineralization and Nitrification in Chinese Fir Plantation

1. Key Laboratory of Humid Subtropical Eco-geographical Process of Ministry of Education, Fuzhou 350007, Fujian, China

2. College of Geographical Sciences, Fujian Normal University, Fuzhou 350007, Fujian, China

Received Date: 2013-04-28

Abstract: In view of the significant role of litter played in regulating forest soil nitrogen transformation, simulating the influence of matrix carbon sources on nitrogen transform in the subtropical coniferous forest acid soil is helpful to deeply understand the mechanism between carbon and nitrogen. The effects of glucose addition (as C of 0, 100, 300, 1 000, 2 000, and 5 000 mg·kg-1) on soil nitrogen content, nitrogen mineralization and nitrification were studied in the soil under subtropical Chinese fir plantation. The results showed that the glucose addition reduced the soil inorganic nitrogen content and proportion in total soluble nitrogen, and the nitrate nitrogen decreased more than ammonium nitrogen; but the decrease of soluble organic nitrogen (SON) and pH was not found, even the ratio of SON in total soluble nitrogen increased. Glucose addition decreased the net nitrogen mineralization and the nitrification rates for nitrogen mineralization were inhibited. The results showed that hydrophilic nitrogen proportion decreased significantly with the addition of glucose, which was related to nitrogen immobilization and transformation to result in the enhancement of SON proportion. Correlation analysis showed that there was a significantly negative linear relationship (R2 = 0.902) between the proportions of nitrate and dissolved organic nitrogen in the total nitrogen of the extract from soil, which indirectly give a evidence of glucose likely promoting the nitrate retaining and transformation by microorganisms involved in soil. The research discovered that the glucose amount (as C) of 1 000 mg ·kg-1 was the threshold affecting the nitrogen transforms in forest soil under subtropical Chinese fir plantation.

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Reference (45)

[1]	Kaiser J. The other global pollutant: nitrogen proves tough to curb [J]. Science, 2001, 294: 268-269
[2]	Bobbink R, Hicks K, Galloway J, et al. Global assessment of nitrogen deposition effects on terrestrial plant diversity: a synthesis [J]. Ecological Applications, 2010, 20: 30-59
[3]	李德军, 莫江明, 方运霆, 等. 模拟氮沉降对南亚热带两种乔木幼苗生物量及其分配的影响[J]. 植物生态学报, 2005, 29 (4):543-549
[4]	郝吉明, 谢绍东, 段雷, 等. 酸沉降临界负荷及应用[M].北京:清华大学出版社, 2001
[5]	Chen X Y,Mulder J. Atmospheric deposition of nitrogen at five subtropical forested sites in South China [J]. Science of the Total Environment, 2007, 378: 317-330
[6]	张乐, 何红波, 章建新, 等. 不同用量葡萄糖对土壤氮素转化的影响[J]. 土壤通报, 2008, 39(4): 775-778
[7]	朱兆良. 农田中氮肥的损失与对策[J]. 土壤与环境, 2000, 9(1): 1-6
[8]	贾俊仙, 李忠佩, 车玉萍. 添加葡萄糖对不同肥力红壤性水稻土氮素转化的影响[J]. 中国农业科学, 2010, 43(8):1617-1624
[9]	贾俊仙, 李忠佩, 车玉萍. 添加葡萄糖对不同肥力黑土氮素转化的影响[J]. 土壤学报, 2011, 48(1): 207-211
[10]	Joergensen R G, Scheu S. Response of soil microorganisms to the addition of carbon, nitrogen and phosphorus in a forest Rendzina[J]. Soil Biology and Biochemistry, 1999, 31(6): 859-866
[11]	Joergensen R G, Raubuch M. Adenylate energy charge of a glucose-treated soil without adding a nitrogen source[J]. Soil Biology and Biochemistry, 2002, 34: 1317-1324
[12]	Yoshitake S, Sasaki A, Uchida M, et al. Carbon and nitrogen limitation to microbial respiration and biomass in an acidic solfatara field[J]. European Journal of Soil Biology, 2007, 43:1-13
[13]	Dendooven L, Alcántara-Hernández R J, Valenzuela-Encinas C, et al. Dynamics of carbon and nitrogen in an extreme alkaline saline soil: A review[J]. Soil Biology and Biochemistry, 2010, 42: 865-877
[14]	Venterea R T, Groffman P M, Verchot L V, et al. Gross nitrogen process rates in temperate forest soils exhibiting symptoms of nitrogen saturation[J]. Forest Ecology and Management, 2004, 196: 129-142
[15]	Szili-Kovács T, Török K, Tilston E L, et al. Promoting microbial immobilization of soil nitrogen during restoration of abandoned agricultural fields by organic additions[J]. Biology of Fertility and Soils, 2007, 43: 823-828
[16]	Chen X Y, Mulder J, Wang Y H, et al. Atmospheric deposition, mineralization and leaching of nitrogen in subtropical forested catchments, South China [J]. Environmental Geochemistry and Health, 2004, 26:179-186
[17]	Bouwman A F, Van Drecht G, Van der Hoek K W. Global and regional surface nitrogen balances in intensive agricultural production systems for the period 1970-2030 [J]. Pedosphere, 2005,15:137-155
[18]	Kanerva S, Smolander A. Microbial activities in forest floor layers under silver birch, Norway spruce and Scots pine [J]. Soil Biology and Biochemistry, 2007,39:1459-1467
[19]	Tan X, Chang S X. Soil compaction and forest litter amendment affect carbon and net nitrogen mineralization in a boreal forest soil [J]. Soil and Tillage Research, 2007, 93:77-86
[20]	Huang Z Q, Xu Z H, Blumfield T J, et al. Soil nitrogen mineralization and fate of (¹⁵NH₄)₂SO₄ in field incubated soil in a hardwood plantation of subtropical Australia: the effect of mulching [J]. Journal of Soils and Sediments, 2008, 8:389-397
[21]	Holub S M, Lajtha K, Spears J D H, et al. Organic matter manipulations have little effect on gross and net nitrogen transformations in two temperate forest mineral soils in the USA and central Europe [J]. Forest Ecology and Management, 2005, 214: 320-330
[22]	Xu X L, Ouyang H, Cao G M. Nitrogen retention patterns and their controlling factors in an alpine meadow: implications for carbon sequestration [J]. Biogeosciences Discuss, 2007,4: 2641-2665
[23]	Ste-Marie C, Houle D. Forest floor gross and net nitrogen mineralization in three forest types in Quebec, Canada [J]. Soil Biology and Biochemistry, 2006, 38: 2135-2143
[24]	向升华, 高人, 马红亮, 等. 不同年龄杉木人工林土壤无机氮比较研究[J]. 亚热带资源与环境学报, 2008, 3(1): 24-30
[25]	Zhang W, Mo J, Yu G, et al. Emissions of nitrous oxide from three tropical forests in Southern China in response to simulated nitrogen deposition [J]. Plant and Soil, 2008, 306:221-236
[26]	Zhao W, Cai Z, Xu Z. Does ammonium-based N addition influence nitrification and acidification in humid subtropical soils of China [J]. Plant Soil, 2007, 297:213-221
[27]	DeForest J L, Zak D R, Pregitzer K S, et al. Atmospheric nitrate deposition, microbial community composition, and enzyme activity in northern hardwood forests [J]. Soil Science Society of America Journal, 2004, 68:132-138
[28]	方运霆, 莫江明, Gundersen P, 等. 森林土壤氮素转换及其对氮沉降的响应[J]. 生态学报, 2004, 24(7): 1523-1531
[29]	邓小文, 韩士杰. 氮沉降对森林生态系统土壤碳库的影响[J].生态学杂志, 2007, 26(10): 1622-1627
[30]	Vesterdal L, Schmidt I K., Callesen I, et al. Carbon and nitrogen in forest floor and mineral soil under six common European tree species[J]. Forest Ecology and Management, 2008, 255: 35-48
[31]	罗献宝,张颖清,徐浩,等. 温带阔叶红松林中不同树种和倒木对土壤性质的影响[J]. 生态环境学报, 2011, 20(12): 1841-1845
[32]	Gilliam F S, Lyttle N L, Thomas A, et al. Soil variability along a nitrogen mineralization and nitrification gradient in a nitrogen-saturated hardwood forest[J]. Soil Science Society of American Journal, 2005,69:247-256
[33]	Magill A H, Aber J D. Variation in soil net mineralization rates with dissolved organic carbon additions[J]. Soil Biology and Biochemistry, 2000, 32:597-601
[34]	Zhang J B, Cai Z C, Zhu T B. N₂O production pathways in the subtropical acid forest soils in China[J]. Environmental Research, 2011,111: 643-649
[35]	钱琛. 亚热带红壤的硝化作用及其对NO₃-N淋溶和土水酸化的影响[D]. 南京: 中国科学院南京土壤研究所, 2008
[36]	俞慎, 李振高. 稻田生态系统生物硝化-反硝化作用与氮素损失[J]. 应用生态学报, 1999, 10 (5) : 630-634
[37]	Lu S, Hu H, Sun Y, et al. Effect of carbon source on the denitrification in constructed wetlands[J]. Journal of Environmental Sciences,2009,21: 1036-1043
[38]	Jordan T E, Weller D E, Correll D L. Denitrification in surface soils of a riparian forest: Effects of water, nitrate and sucrose additions[J]. Soil Biology and Biochemistry, 1998, 30:833-843
[39]	葛晓改, 肖文发, 曾立雄, 等. 不同林龄马尾松凋落物基质质量与土壤养分的关系[J]. 生态学报, 2012, 32(3) : 852-862
[40]	刘维丽, 马红亮, 彭秀明, 等. 凋落物中次生代谢物对森林土壤可溶性氮的影响[J]. 土壤, 2010,42(4):564-568
[41]	王杰, 马红亮, 高人, 等. 添加酚类物质对森林土壤无机氮的影响[J].安徽农业科学, 2010, 38(10): 5184-5187
[42]	马红亮, 刘维丽, 高人, 等. 凋落物与单宁酸对森林土壤无机氮的影响[J].应用生态学报, 2011, 22(1): 61-65
[43]	Dodla S K, Wang J J, DeLaune R D, et al. Denitrification potential and its relation to organic carbon quality in three coastal wetland soils[J]. Science of the Total Environment, 2008, 407: 471-480
[44]	Ma H L, Gao R, Wang J, et al. Response of soil NH₄⁺ and NO₃^- transformations to nitrogen deposition under addition of litters and tannin [M]// Li S C, Wang W Y, Niu P H, et al. Progress in Environmental Science and Technology (VOL.Ⅲ) .Beijing Jiaotong University, Science Press, 2011, 54-59
[45]	Lummer D, Scheu S, Butenschoen O. Connecting litter quality, microbial community and nitrogen transfer mechanisms in decomposing litter mixtures [J]. Oikos, 2012, 121: 1649-1655

Effects of Glucose Addition on Soil Nitrogen Transformation and Net Mineralization and Nitrification in Chinese Fir Plantation

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通讯作者: 陈斌, bchen63@163.com

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Effects of Glucose Addition on Soil Nitrogen Transformation and Net Mineralization and Nitrification in Chinese Fir Plantation

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