cDNA-AFLP Analysis of Differentially Expressed Transcripts in Early and Middle Development Stages of Male Inflorescence in White Birch(Betula platyphylla Suk.)

BAO Shuai; KONG Xue; XING Lei; ZHANG Cui-xia; GUAN Min-xiao; ZHAO Li-hong; LIU Chuang; LIU Xue-mei

Volume 27 Issue 4

Article Contents

Turn off MathJax

Article Navigation > Forest Research > 2014 > 27(4): 466-473

Citation:

cDNA-AFLP Analysis of Differentially Expressed Transcripts in Early and Middle Development Stages of Male Inflorescence in White Birch(Betula platyphylla Suk.)

1.
College of Life Science, Northeast Forestry University, Harbin 150040, Heilongjiang, China
2.
Forestry Station of Xiangcheng Town, Zoucheng 273503, Shandong, China

Received Date: 2013-11-05

Abstract

The reproductive development of male inflorescence for Betula platyphylla takes almost a whole year from the emergence of original male inflorescence to the formation of mature pollen. The early and middle development periods are much essential for the development of male gametophyte of B. platyphylla. The differentially expressed transcripts in early and middle developmental stages were analyzed by cDNA-Amplified Fragment Length Polymorphism (cDNA-AFLP) technique. Among 62 successfully sequenced fragments, 30 showed significant homologies to known genes in Genbank, while the other 32 were supposed to unknown and new sequences. GO annotation showed that these known genes were involved in the processes of metabolic process, cellular process, response to stimulus, reproduction, signal transduction, and biological regulation, and then with the molecular function of catalytic activity, binding molecular, enzyme regulator activity and transporter activity. Two important TDFs involved in reproduction and male inflorescence development of B. platyphylla were found, named as Bplbs658 and Bplbs199. In addition, the transcript expression of 11 TDFs were examined in different tissues by qRT-PCR. The results indicated that these genes were involved in the development of both floral tissues and vegetative tissues of B. platyphylla with distinct tissue-specific expression patterns.
- Betula platyphylla,
- reproduction,
- differential expression,
- transcription,
- floral development,
- male inflorescence

References

[1]	Coen E S, Meyerowitz E M. The war of the whorls: Genetics interactions controlling flower development [J]. Nature,1991,353(6339):31-37
[2]	Shaogui G, Yi Z, Je-Gun J, et al. Transcriptome sequencing and comparative analysis of cucumber flowers with different sex types [J]. BMC Genomics, 2010, 11: 384-397
[3]	David H, David T. Transcriptome analysis of haploid male gametophyte development in Arabidopsis[J]. Genome Biol, 2004, 5(11): R85
[4]	Rebecca M D, Candice N H, Malali G, et al. Utility of RNA sequencing for analysis of maize reproductive transcriptomes [J]. The Plant Genome, 2011, 4(3): 191-203
[5]	Ma H. Molecular genetic analyses of microsporogenesis microgametogenesis in flowering plants[J]. Annu Rev Plant Biol,2005,56:393-434
[6]	Chang F,Wang Y, Wang S, et al. Molecular control of microsporogenesis in Arabidopsis [J]. Current Opinion in Plant Biology,2011,14(1):66-73
[7]	Li J, Yu M, Geng L L, et al. The fasciclin-like arabinogalactan protein gene,FLA3,is involved in microspore development of Arabidopsis[J]. The Plant Journal,2010,64(3):482-497
[8]	Xu J, Yang C, Yuan Z, et al. The ABORTED MICR-OSPORES regulatory network is required for postmeiotic male reproductive development in Arabidopsis thaliana[J]. The Plant Cell,2010,22(1):91-107
[9]	Sorensen A M, Krober S, Unte U S, et al. The Arabidopsis ABORTED MICROSPORES (AMS) gene encodes a MYC class transcription factor [J]. The Plant Journal,2003,33(2):413-423
[10]	姜立杰,张开春,张晓明. cDNA-AFLP 技术及其在基因表达研究中的应用[J].中国生物工程杂志,2003,23(12):82-86
[11]	Jones J T, Harrower B E. A comparison of the efficiency of differential display and cDNA-AFLPs as tools for the isolation of differentially expressed parasite genes [J]. Fundamental and Applied Nematology,1998,21(1):81-88
[12]	曾凡锁,南楠,詹亚光. 富含多糖和次生代谢产物的白桦成熟叶中总RNA的提取[J].植物生理学通讯, 2007,43(5):913-916
[13]	Bachem C W, Oomen R J F, Visser R G. Transript imaging with cDNA-AFLP: a step-by-step protocol [J]. Plant Molecular Biology Reporter,1998,16(2):157-173
[14]	邢磊,刘雪梅,宋福南. 白桦 cDNA-AFLP 体系的优化和建立[J].东北林业大学学报,2010,38(10):15-18
[15]	Altschul S F, Madden T L, Schaffer A A, et al. Gapped BLAST and PSI-BLAST:A new generation of protein database search programs [J]. Nucleic Acids Res,1997,25(17):3389-3402
[16]	Heslop-Harrison J: The experimental modification of sex expression in flowering plants[J]. Biological Reviews, 1957, 32(1):38-90
[17]	Korpelainen H: Labile sex expression in plants [J]. Biological Reviews 1998, 73(2):157-180
[18]	Aifen Z, Huachun W, John C. W, et al. BRL1, a leucine-rich repeat receptor-like protein kinase, is functionally redundant with BRI1 in regulating Arabidopsis brassinosteroid signaling [J]. The Plant Journal, 2004, 40(3):399-409
[19]	马媛媛,甘睿,王宁宁. 植物富含亮氨酸重复序列型类受体蛋白激酶的生物学功能 [J]. 植物生理与分子生物学学报, 2005, 31 (4): 331-339
[20]	Liu H Y, Toyn, J H, Chiang Y C, et al. DBF2, a cell cycle-regulated protein kinase, is physically and functionally associated with the CCR4 transcriptional regulatory complex[J]. EMBO J. 1997,16:5289-5298
[21]	Berthet C, Morera A M, Asensio M J, et al. CCR4-associated factor CAF1 is an essential factor for spermatogenesis [J].Molecular and Cellular Biology, 2004,24: 5808-5820
[22]	Bleecker A B, Kende H. Ethylene:a gaseous signal molecule in plants [J]. Annual Review of Cell and Developmental Biology,2000,16(1):1-18
[23]	Ju C, Yoon G M, Shemansky J M, et al. CTR1 phosphorylates the central regulator EIN2 to control ethylene hormone signaling from the ER membrane to the nucleus in Arabidopsis [J]. Proceedings of the National Academy of Sciences,2012, 109(47):19486-19491
[24]	Alonso J M, Ecker J R. The ethylene pathway:a paradigm for plant hormone signaling and interaction [J]. Science Signaling,2001,(70):re1
[25]	Qiao H, Shen Z, Huang S C, et al. Processing and subcellular trafficking of ER-tethered EIN2 control response to ethylene gas [J]. Science Signaling,2012,338(6105):390-393
[26]	Wang J, Chen G, Hu Z, et al. Clonging and charact-erization of the EIN2 homology gene LeEIN2 from tomato:full length research article [J]. Mitochondrial DNA,2007,18(1):33-38
[27]	Voet D, Voet J G, Pratt C W. Electron transport and oxidative phosphorylation [M]. Biochemistry, 3rd ed. Hoboken,NJ:John Wiley & Sons,2004:797-842
[28]	Campbell M K, Farrell S O. Bioquímica [M]. Cengage Learning Latin America,2004:515-543
[29]	Araujo W L, Nunes-nesi A, Nikoloski Z, et al. Metabolic control and regulation of the tricarboxylic acid cycle in photosynthetic and heterotrophic plant tissues [J]. Plant ,Cell & Environment,2012,35(1):1-21
[30]	Carlsson J, Leino M, Sohlberg J, et al. Mitoch-ondrial regulation of flower development [J]. Mitoch-. ondrion,2008,8:74-86
[31]	Araujo W L, Tohge T, Nunes-nesi A, et al. Phosphonate analogs of 2-oxoglutarate perturb metabolism and gene expression in illuminated Arabidopsis leaves [J]. Frontiers in Plant Science, 2012, 114:1-44
[32]	Hubbard P A, Liang X, Schulz H, et al. The crystal structure and reaction mechanism of Escherichia coli 2,4-dienoyl-CoA reductase [J]. Journal of Biological Chemistry,2003,278(39):37553-37560
[33]	Lyons J M. Chilling injury in plants[J]. Annual Review of Plant Physiology,1973,24(1):445-466
[34]	Kohzuma K, Dal B C, Kanazawa A, et al. Thioredoxin-insensitive plastid ATP synthase that performs moonlighting functions [J]. Proceedings of the National Academy of Sciences, 2012,109(9):3293-3298
[35]	Li W Q, Zhang X Q, Xia C, et al. Male Game-Gametophyte Defective 1,encoding the Fad subunit of mitochondrial F1F0-ATP synthase, is essential for pollen formation in Arabidopsis thaliana[J]. Plant and Cell Physiology,2010,51(6):923-935

Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Get Citation

PDF

XML

Article views(2878) PDF downloads(1100) Cited by()

Proportional views

cDNA-AFLP Analysis of Differentially Expressed Transcripts in Early and Middle Development Stages of Male Inflorescence in White Birch(Betula platyphylla Suk.)

1. College of Life Science, Northeast Forestry University, Harbin 150040, Heilongjiang, China

2. Forestry Station of Xiangcheng Town, Zoucheng 273503, Shandong, China

Received Date: 2013-11-05

Abstract: The reproductive development of male inflorescence for Betula platyphylla takes almost a whole year from the emergence of original male inflorescence to the formation of mature pollen. The early and middle development periods are much essential for the development of male gametophyte of B. platyphylla. The differentially expressed transcripts in early and middle developmental stages were analyzed by cDNA-Amplified Fragment Length Polymorphism (cDNA-AFLP) technique. Among 62 successfully sequenced fragments, 30 showed significant homologies to known genes in Genbank, while the other 32 were supposed to unknown and new sequences. GO annotation showed that these known genes were involved in the processes of metabolic process, cellular process, response to stimulus, reproduction, signal transduction, and biological regulation, and then with the molecular function of catalytic activity, binding molecular, enzyme regulator activity and transporter activity. Two important TDFs involved in reproduction and male inflorescence development of B. platyphylla were found, named as Bplbs658 and Bplbs199. In addition, the transcript expression of 11 TDFs were examined in different tissues by qRT-PCR. The results indicated that these genes were involved in the development of both floral tissues and vegetative tissues of B. platyphylla with distinct tissue-specific expression patterns.

HTML

Reference (35)

[1]	Coen E S, Meyerowitz E M. The war of the whorls: Genetics interactions controlling flower development [J]. Nature,1991,353(6339):31-37
[2]	Shaogui G, Yi Z, Je-Gun J, et al. Transcriptome sequencing and comparative analysis of cucumber flowers with different sex types [J]. BMC Genomics, 2010, 11: 384-397
[3]	David H, David T. Transcriptome analysis of haploid male gametophyte development in Arabidopsis[J]. Genome Biol, 2004, 5(11): R85
[4]	Rebecca M D, Candice N H, Malali G, et al. Utility of RNA sequencing for analysis of maize reproductive transcriptomes [J]. The Plant Genome, 2011, 4(3): 191-203
[5]	Ma H. Molecular genetic analyses of microsporogenesis microgametogenesis in flowering plants[J]. Annu Rev Plant Biol,2005,56:393-434
[6]	Chang F,Wang Y, Wang S, et al. Molecular control of microsporogenesis in Arabidopsis [J]. Current Opinion in Plant Biology,2011,14(1):66-73
[7]	Li J, Yu M, Geng L L, et al. The fasciclin-like arabinogalactan protein gene,FLA3,is involved in microspore development of Arabidopsis[J]. The Plant Journal,2010,64(3):482-497
[8]	Xu J, Yang C, Yuan Z, et al. The ABORTED MICR-OSPORES regulatory network is required for postmeiotic male reproductive development in Arabidopsis thaliana[J]. The Plant Cell,2010,22(1):91-107
[9]	Sorensen A M, Krober S, Unte U S, et al. The Arabidopsis ABORTED MICROSPORES (AMS) gene encodes a MYC class transcription factor [J]. The Plant Journal,2003,33(2):413-423
[10]	姜立杰,张开春,张晓明. cDNA-AFLP 技术及其在基因表达研究中的应用[J].中国生物工程杂志,2003,23(12):82-86
[11]	Jones J T, Harrower B E. A comparison of the efficiency of differential display and cDNA-AFLPs as tools for the isolation of differentially expressed parasite genes [J]. Fundamental and Applied Nematology,1998,21(1):81-88
[12]	曾凡锁,南楠,詹亚光. 富含多糖和次生代谢产物的白桦成熟叶中总RNA的提取[J].植物生理学通讯, 2007,43(5):913-916
[13]	Bachem C W, Oomen R J F, Visser R G. Transript imaging with cDNA-AFLP: a step-by-step protocol [J]. Plant Molecular Biology Reporter,1998,16(2):157-173
[14]	邢磊,刘雪梅,宋福南. 白桦 cDNA-AFLP 体系的优化和建立[J].东北林业大学学报,2010,38(10):15-18
[15]	Altschul S F, Madden T L, Schaffer A A, et al. Gapped BLAST and PSI-BLAST:A new generation of protein database search programs [J]. Nucleic Acids Res,1997,25(17):3389-3402
[16]	Heslop-Harrison J: The experimental modification of sex expression in flowering plants[J]. Biological Reviews, 1957, 32(1):38-90
[17]	Korpelainen H: Labile sex expression in plants [J]. Biological Reviews 1998, 73(2):157-180
[18]	Aifen Z, Huachun W, John C. W, et al. BRL1, a leucine-rich repeat receptor-like protein kinase, is functionally redundant with BRI1 in regulating Arabidopsis brassinosteroid signaling [J]. The Plant Journal, 2004, 40(3):399-409
[19]	马媛媛,甘睿,王宁宁. 植物富含亮氨酸重复序列型类受体蛋白激酶的生物学功能 [J]. 植物生理与分子生物学学报, 2005, 31 (4): 331-339
[20]	Liu H Y, Toyn, J H, Chiang Y C, et al. DBF2, a cell cycle-regulated protein kinase, is physically and functionally associated with the CCR4 transcriptional regulatory complex[J]. EMBO J. 1997,16:5289-5298
[21]	Berthet C, Morera A M, Asensio M J, et al. CCR4-associated factor CAF1 is an essential factor for spermatogenesis [J].Molecular and Cellular Biology, 2004,24: 5808-5820
[22]	Bleecker A B, Kende H. Ethylene:a gaseous signal molecule in plants [J]. Annual Review of Cell and Developmental Biology,2000,16(1):1-18
[23]	Ju C, Yoon G M, Shemansky J M, et al. CTR1 phosphorylates the central regulator EIN2 to control ethylene hormone signaling from the ER membrane to the nucleus in Arabidopsis [J]. Proceedings of the National Academy of Sciences,2012, 109(47):19486-19491
[24]	Alonso J M, Ecker J R. The ethylene pathway:a paradigm for plant hormone signaling and interaction [J]. Science Signaling,2001,(70):re1
[25]	Qiao H, Shen Z, Huang S C, et al. Processing and subcellular trafficking of ER-tethered EIN2 control response to ethylene gas [J]. Science Signaling,2012,338(6105):390-393
[26]	Wang J, Chen G, Hu Z, et al. Clonging and charact-erization of the EIN2 homology gene LeEIN2 from tomato:full length research article [J]. Mitochondrial DNA,2007,18(1):33-38
[27]	Voet D, Voet J G, Pratt C W. Electron transport and oxidative phosphorylation [M]. Biochemistry, 3rd ed. Hoboken,NJ:John Wiley & Sons,2004:797-842
[28]	Campbell M K, Farrell S O. Bioquímica [M]. Cengage Learning Latin America,2004:515-543
[29]	Araujo W L, Nunes-nesi A, Nikoloski Z, et al. Metabolic control and regulation of the tricarboxylic acid cycle in photosynthetic and heterotrophic plant tissues [J]. Plant ,Cell & Environment,2012,35(1):1-21
[30]	Carlsson J, Leino M, Sohlberg J, et al. Mitoch-ondrial regulation of flower development [J]. Mitoch-. ondrion,2008,8:74-86
[31]	Araujo W L, Tohge T, Nunes-nesi A, et al. Phosphonate analogs of 2-oxoglutarate perturb metabolism and gene expression in illuminated Arabidopsis leaves [J]. Frontiers in Plant Science, 2012, 114:1-44
[32]	Hubbard P A, Liang X, Schulz H, et al. The crystal structure and reaction mechanism of Escherichia coli 2,4-dienoyl-CoA reductase [J]. Journal of Biological Chemistry,2003,278(39):37553-37560
[33]	Lyons J M. Chilling injury in plants[J]. Annual Review of Plant Physiology,1973,24(1):445-466
[34]	Kohzuma K, Dal B C, Kanazawa A, et al. Thioredoxin-insensitive plastid ATP synthase that performs moonlighting functions [J]. Proceedings of the National Academy of Sciences, 2012,109(9):3293-3298
[35]	Li W Q, Zhang X Q, Xia C, et al. Male Game-Gametophyte Defective 1,encoding the Fad subunit of mitochondrial F1F0-ATP synthase, is essential for pollen formation in Arabidopsis thaliana[J]. Plant and Cell Physiology,2010,51(6):923-935

cDNA-AFLP Analysis of Differentially Expressed Transcripts in Early and Middle Development Stages of Male Inflorescence in White Birch(Betula platyphylla Suk.)

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Proportional views

cDNA-AFLP Analysis of Differentially Expressed Transcripts in Early and Middle Development Stages of Male Inflorescence in White Birch(Betula platyphylla Suk.)

HTML

Catalog

Export File

Citation

Format

Content