The Effects of AtFBDL1 on Apical Meristematic Growth of Arabidopsis thaliana

LI Jian-bo; ZHANG Jin; LIU Bo-bin; XU You-ming; LU Meng-zhu; CHEN Jun

Volume 28 Issue 1

Article Contents

Turn off MathJax

Article Navigation > Forest Research > 2015 > 28(1): 1-7

Citation:

The Effects of AtFBDL1 on Apical Meristematic Growth of Arabidopsis thaliana

1.
State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China
2.
College of Horticulture & Forestry Sciences, Huazhong Agricultural University, Wuhan 430000, Hubei, China

Received Date: 2013-04-09

Abstract

AtFBDL1 is a member of FBD-like gene family, which encodes proteins containing sequences similar to F-box structure domain. Online expression prediction indicates that AtFBDL1 is mainly expressed in shoot apical meristem. Few studies have been conducted on FBD-like genes, which cause the function of these genes remains largely unknown. By using tissue-specific semi-quantitative analysis and GUS staining, the temporospatial expression pattern of AtFBDL1 in Arabidopsis thaliana was investigated. The result showed that in the early stage of seedling development, the AtFBDL1 was mainly expressed in shoot apical meristem and in hypocotyl. After the stage of euphylla emerged, the expression of AtFBDL1 in hypocotyl decreased significantly and mainly restricted the shoot apical meristem. Compared with wild type plants, the AtFBDL1 over-expressed plants displayed slowed growth with up to 12 cm shorter stems, delayed bolting time of 3 to 4 days, reduced rosette leaf area, increased leaf number up to 10, and abnormal leaves. Co-expression analysis showed that the transcription of AtFBDL1 was correlated with several auxin-related or flowering regulation genes. The result of this study indicates that the AtFBDL1 plays an essential role in plant growth and development in Arabidopsis thaliana, especially in the process of the shoot apical meristem differentiation, highly likely through auxin-related pathways.
- Arabidopsis thaliana,
- F-box domain,
- gene function,
- shoot apical meristem

References

[1]	Steeves T A, Sussex I M. Patterns in plant development[M]. Cambridge:Syndicate of the university of Cambridge, 1989: 6-10.
[2]	Takacs E M, Du C, Ponnala L, et al. Ontogeny of the Maize Shoot Apical Meristem[J]. The Plant Cell, 2012, 24(8): 3219-3234.
[3]	Barton M. Twenty years on: the inner workings of the shoot apical meristem, a developmental dynamo[J]. Developmental biology, 2010, 341(1): 95-113.
[4]	Bowman J L, Eshed Y. Formation and maintenance of the shoot apical meristem[J]. Trends in plant science, 2000, 5(3): 110-115.
[5]	王占军, 陈金慧, 施季森. 植物干细胞中WUS/CLV反馈调控机制的研究进展[J]. 林业科学, 2011, 47(4): 159-165.
[6]	姜妍, 祖伟, 吴存祥. 茎顶端分生组织在植物发育过程中的保持, 转变和逆转[J]. 细胞生物学杂志, 2008, 30(2): 147-152.
[7]	Endrizzi K, Moussian B, Haecker A, et al. The SHOOT MERISTEMLESS gene is required for maintenance of undifferentiated cells in Arabidopsis shoot and floral meristems and acts at a different regulatory level than the meristem genes WUSCHEL and ZWILLE[J]. The Plant Journal, 1996, 10(6): 967-979.
[8]	Ung N, Lal S, Smith H M S. The Role of PENNYWISE and POUND-FOOLISH in the Maintenance of the Shoot Apical Meristem in Arabidopsis[J]. Plant physiology, 2011, 156(2): 605-614.
[9]	Hibara K I, Takada S, Tasaka M. CUC1 gene activates the expression of SAM-related genes to induce adventitious shoot formation[J]. The Plant Journal, 2003, 36(5): 687-696.
[10]	Schultz E A, Haughn G W. LEAFY, a homeotic gene that regulates inflorescence development in Arabidopsis[J]. The Plant Cell, 1991, 3(8): 771-781.
[11]	Yadav R K, Girke T, Pasala S, et al. Gene expression map of the Arabidopsis shoot apical meristem stem cell niche[J]. Proceedings of the National Academy of Sciences, 2009, 106(12): 4941-4946.
[12]	徐芳, 熊爱生, 彭日荷, 等. 植物遗传转化的新方法[J]. 中国蔬菜, 2005,1(3):29-31.
[13]	Clough S J, Bent A F. Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana[J]. The Plant Journal, 1998, 16(6): 735-743.
[14]	Cecchetti V, Pomponi M, Altamura M M, et al. Expression of rolB in tobacco flowers affects the coordinated processes of anther dehiscence and style elongation[J]. The Plant Journal, 2004, 38(3): 512-525.
[15]	Kipreos E T, Pagano M. The F-box protein family[J]. Genome Biol, 2000, 1(5): 1-7.
[16]	Xu G, Ma H, Nei M, et al. Evolution of F-box genes in plants: different modes of sequence divergence and their relationships with functional diversification[J]. Proceedings of the National Academy of Sciences, 2009, 106(3): 835-840.
[17]	Dharmasiri N, Dharmasiri S, Weijers D, et al. Plant development is regulated by a family of auxin receptor F-box proteins[J]. Developmental cell, 2005, 9(1): 109-119.
[18]	Kepinski S, Leyser O. The Arabidopsis F-box protein TIR1 is an auxin receptor[J]. Nature, 2005, 435(7041): 446-451.
[19]	Benková E, Michniewicz M, Sauer M, et al. Local, efflux-dependent auxin gradients as a common module for plant organ formation[J]. Cell, 2003, 115(5): 591-602.
[20]	Spinelli S V, Martin A P, Viola I L, et al. A mechanistic link between STM and CUC1 during Arabidopsis development[J]. Plant physiology, 2011, 156(4): 1894-1904.
[21]	Xu L, Xu Y, Dong A, et al. Novel as1 and as2 defects in leaf adaxial-abaxial polarity reveal the requirement for ASYMMETRIC LEAVES1 and 2 and ERECTA functions in specifying leaf adaxial identity[J]. Development, 2003, 130(17): 4097-4107.
[22]	Coen E S, Meyerowitz E M. The war of the whorls: genetic interactions controlling flower development[J]. Nature, 1991, 353(6339): 31-37.
[23]	Ahn J H, Miller D, Winter V J, et al. A divergent external loop confers antagonistic activity on floral regulators FT and TFL1[J]. The EMBO journal, 2006, 25(3): 605-614.

Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Get Citation

PDF

XML

Article views(3131) PDF downloads(1448) Cited by()

Proportional views

The Effects of AtFBDL1 on Apical Meristematic Growth of Arabidopsis thaliana

1. State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China

2. College of Horticulture & Forestry Sciences, Huazhong Agricultural University, Wuhan 430000, Hubei, China

Received Date: 2013-04-09

Abstract: AtFBDL1 is a member of FBD-like gene family, which encodes proteins containing sequences similar to F-box structure domain. Online expression prediction indicates that AtFBDL1 is mainly expressed in shoot apical meristem. Few studies have been conducted on FBD-like genes, which cause the function of these genes remains largely unknown. By using tissue-specific semi-quantitative analysis and GUS staining, the temporospatial expression pattern of AtFBDL1 in Arabidopsis thaliana was investigated. The result showed that in the early stage of seedling development, the AtFBDL1 was mainly expressed in shoot apical meristem and in hypocotyl. After the stage of euphylla emerged, the expression of AtFBDL1 in hypocotyl decreased significantly and mainly restricted the shoot apical meristem. Compared with wild type plants, the AtFBDL1 over-expressed plants displayed slowed growth with up to 12 cm shorter stems, delayed bolting time of 3 to 4 days, reduced rosette leaf area, increased leaf number up to 10, and abnormal leaves. Co-expression analysis showed that the transcription of AtFBDL1 was correlated with several auxin-related or flowering regulation genes. The result of this study indicates that the AtFBDL1 plays an essential role in plant growth and development in Arabidopsis thaliana, especially in the process of the shoot apical meristem differentiation, highly likely through auxin-related pathways.

HTML

Reference (23)

[1]	Steeves T A, Sussex I M. Patterns in plant development[M]. Cambridge:Syndicate of the university of Cambridge, 1989: 6-10.
[2]	Takacs E M, Du C, Ponnala L, et al. Ontogeny of the Maize Shoot Apical Meristem[J]. The Plant Cell, 2012, 24(8): 3219-3234.
[3]	Barton M. Twenty years on: the inner workings of the shoot apical meristem, a developmental dynamo[J]. Developmental biology, 2010, 341(1): 95-113.
[4]	Bowman J L, Eshed Y. Formation and maintenance of the shoot apical meristem[J]. Trends in plant science, 2000, 5(3): 110-115.
[5]	王占军, 陈金慧, 施季森. 植物干细胞中WUS/CLV反馈调控机制的研究进展[J]. 林业科学, 2011, 47(4): 159-165.
[6]	姜妍, 祖伟, 吴存祥. 茎顶端分生组织在植物发育过程中的保持, 转变和逆转[J]. 细胞生物学杂志, 2008, 30(2): 147-152.
[7]	Endrizzi K, Moussian B, Haecker A, et al. The SHOOT MERISTEMLESS gene is required for maintenance of undifferentiated cells in Arabidopsis shoot and floral meristems and acts at a different regulatory level than the meristem genes WUSCHEL and ZWILLE[J]. The Plant Journal, 1996, 10(6): 967-979.
[8]	Ung N, Lal S, Smith H M S. The Role of PENNYWISE and POUND-FOOLISH in the Maintenance of the Shoot Apical Meristem in Arabidopsis[J]. Plant physiology, 2011, 156(2): 605-614.
[9]	Hibara K I, Takada S, Tasaka M. CUC1 gene activates the expression of SAM-related genes to induce adventitious shoot formation[J]. The Plant Journal, 2003, 36(5): 687-696.
[10]	Schultz E A, Haughn G W. LEAFY, a homeotic gene that regulates inflorescence development in Arabidopsis[J]. The Plant Cell, 1991, 3(8): 771-781.
[11]	Yadav R K, Girke T, Pasala S, et al. Gene expression map of the Arabidopsis shoot apical meristem stem cell niche[J]. Proceedings of the National Academy of Sciences, 2009, 106(12): 4941-4946.
[12]	徐芳, 熊爱生, 彭日荷, 等. 植物遗传转化的新方法[J]. 中国蔬菜, 2005,1(3):29-31.
[13]	Clough S J, Bent A F. Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana[J]. The Plant Journal, 1998, 16(6): 735-743.
[14]	Cecchetti V, Pomponi M, Altamura M M, et al. Expression of rolB in tobacco flowers affects the coordinated processes of anther dehiscence and style elongation[J]. The Plant Journal, 2004, 38(3): 512-525.
[15]	Kipreos E T, Pagano M. The F-box protein family[J]. Genome Biol, 2000, 1(5): 1-7.
[16]	Xu G, Ma H, Nei M, et al. Evolution of F-box genes in plants: different modes of sequence divergence and their relationships with functional diversification[J]. Proceedings of the National Academy of Sciences, 2009, 106(3): 835-840.
[17]	Dharmasiri N, Dharmasiri S, Weijers D, et al. Plant development is regulated by a family of auxin receptor F-box proteins[J]. Developmental cell, 2005, 9(1): 109-119.
[18]	Kepinski S, Leyser O. The Arabidopsis F-box protein TIR1 is an auxin receptor[J]. Nature, 2005, 435(7041): 446-451.
[19]	Benková E, Michniewicz M, Sauer M, et al. Local, efflux-dependent auxin gradients as a common module for plant organ formation[J]. Cell, 2003, 115(5): 591-602.
[20]	Spinelli S V, Martin A P, Viola I L, et al. A mechanistic link between STM and CUC1 during Arabidopsis development[J]. Plant physiology, 2011, 156(4): 1894-1904.
[21]	Xu L, Xu Y, Dong A, et al. Novel as1 and as2 defects in leaf adaxial-abaxial polarity reveal the requirement for ASYMMETRIC LEAVES1 and 2 and ERECTA functions in specifying leaf adaxial identity[J]. Development, 2003, 130(17): 4097-4107.
[22]	Coen E S, Meyerowitz E M. The war of the whorls: genetic interactions controlling flower development[J]. Nature, 1991, 353(6339): 31-37.
[23]	Ahn J H, Miller D, Winter V J, et al. A divergent external loop confers antagonistic activity on floral regulators FT and TFL1[J]. The EMBO journal, 2006, 25(3): 605-614.

The Effects of AtFBDL1 on Apical Meristematic Growth of Arabidopsis thaliana

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Proportional views

The Effects of AtFBDL1 on Apical Meristematic Growth of Arabidopsis thaliana

HTML

Catalog

Export File

Citation

Format

Content