Interacting Proteins of Tamarix hispida Translation Initiation Factor 1A and Their Expression Pattern Analysis

ZHAO Zhen; YANG Gui-yan; ZHANG Feng-jiao; GAO Cai-qiu

Volume 28 Issue 1

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Interacting Proteins of Tamarix hispida Translation Initiation Factor 1A and Their Expression Pattern Analysis

1.
State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, Heilongjiang, China

Received Date: 2014-04-15

Abstract

Five different proteins which interacted with Tamarix hispida translation initiation factor (TheIF1A) gene were obtained by yeast two-hybrid assay. They were RNA polymerase beta II subunit, ATP synthase CF1 alpha subunit protein, cytochrome b6/f complex subunit IV, ribulose 1, 5-bisphosphate carboxylase/oxygenase small subunit and histone acetyltransferase. Furthermore, the expression patterns of these proteins and TheIF1A under normal and abiotic stresses were analyzed by RT-PCR analysis. The results showed that these interacting proteins were all shared similar expression patterns with TheIF1A, suggesting that these proteins may act together to regulate the stress tolerance of T. hispida.
- Tamarix hispida,
- yeast two-hybrid,
- interacting protein,
- translation initiation factor 1A (eIF1A),
- stress tolerance

References

[1]	Holcik M, N. Sonenberg. Translational Control in Stress and Apoptosis[J]. Nature Reviews Molecular Cell Biology. 2005, 6 (4): 318-327.
[2]	Howarth C. Molecular Responses of Plants to an Increased Incidence of Heat Shock[J]. Plant, Cell & Environment. 1991, 14 (8): 831-841.
[3]	Dong Z, Zhang J. Initiation Factor eIF3 and Regulation of mRNA Translation, Cell Growth, and Cancer[J]. Critical Reviews in Oncology/Hematology. 2006, 59 (3): 169-180.
[4]	Pyronnet S, Sonenberg N. Cell-Cycle-Dependent Translational Control[J]. Current Opinion in Genetics & Development. 2001, 11 (1): 13-18.
[5]	Kyrpides N C, Woese C R. Universally Conserved Translation Initiation Factors[J]. Proceedings of the National Academy of Sciences. 1998, 95 (1): 224-228.
[6]	Mecak A R, Shin B S, Dever T E, et al. Engaging the Ribosome: Universal IFs of Translation[J]. Trends in Biochemical Sciences. 2001, 26 (12): 705-709.
[7]	Olsen D S, Savner E M, Mathew A, et al. Domains of eIF1A That Mediate Binding to eIF2, eIF3 and eIF5b and Promote Ternary Complex Recruitment in Vivo[J]. The EMBO Journal. 2003, 22 (2): 193-204.
[8]	Rausell A, Kanhonou R, Yenush L, et al. The Translation Initiation Factor eIF1A Is an Important Determinant in the Tolerance to NaCl Stress in Yeast and Plants[J]. The Plant Journal. 2003, 34 (3): 257-267.
[9]	王留强, 柽柳真核起始因子 5A (elFSA) 基因的功能研究 [D].东北林业大学硕士论文. 2011.
[10]	Taylor D N, Carr JP. The Gcd10 Subunit of Yeast eIF-3 Binds the Methyltransferase-Like Domain of the 126 and 183 Kda Replicase Proteins of Tobacco Mosaic Virus in the Yeast Two-Hybrid System[J]. Journal of General Virology. 2000, 81 (6): 1587-1591.
[11]	Stebbins-Boaz B, Cao Q, de Moor C H, et al. Maskin Is a CPEB-Associated Factor That Transiently Interacts with eIF-4e[J]. Molecular Cell. 1999, 4 (6): 1017-1027.
[12]	于丽丽. 柽柳 ThDREB 和 TheIF5A 基因抗逆功能研究 [D]. 东北林业大学, 2011.
[13]	管蕊. 利用酵母双杂交系统筛选与 BMNPVPE38 和 VIF-1 相互作用的蛋白 [D]. 江苏科技大学, 2012.
[14]	Livak K J, Schmittgen T D. Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2^-ΔΔC_T Method[J]. Methods (San Diego, Calif.). 2001, 25 (4): 402-408.
[15]	Battiste J L, Pestova T V, Hellen C U, et al. The eIF1A Solution Structure Reveals a Large RNA-Binding Surface Important for Scanning Function[J]. Molecular Cell. 2000, 5 (1): 109-119.
[16]	Chaudhuri J, Si K, Maitra U. Function of Eukaryotic Translation Initiation Factor 1A (eIF1A) (Formerly Called eIF-4c) in Initiation of Protein Synthesis[J]. Journal of Biological Chemistry. 1997, 272 (12): 7883-7891.
[17]	Tezara W, Mitchell V, Driscoll S, et al. Water Stress Inhibits Plant Photosynthesis by Decreasing Coupling Factor and ATP[J]. Nature. 1999, 401 (6756): 914-917.
[18]	Niu X, Bressan RA, Hasegawa P M,et al. Ion Homeostasis in NaCl Stress Environments[J]. Plant Physiology. 1995, 109 (3): 735.
[19]	Wang W, Vinocur B, Altman A. Plant Responses to Drought, Salinity and Extreme Temperatures: Towards Genetic Engineering for stress tolerance[J]. Planta. 2003, 218 (1): 1-14.
[20]	毛海滨, 李国富, 阮翔, 等. 质体库和细胞色素 B6/f 参与调控蓝细菌 (Synechocystis Sp. Pcc 6803) 的状态转换[J]. Acta Biophysica Sinica. 2002, 18 (3).
[21]	施定基, 张超, 李世明, 等. 蓝藻与植物叶绿体光合系统基因的生物信息学研究[J]. 遗传学报. 2004, 31 (6): 627-633.
[22]	梅杨, 李海蓝, 谢晋, 等. 核酮糖-1, 5-二磷酸羧化酶/加氧酶 (Rubisco)[J]. 植物生理学通讯. 2007, 43 (2): 363-368.
[23]	刘拥海, 彭新湘, 李明启. 水稻叶片中过氧化氢与核酮糖-1, 5-二磷酸羧化酶/加氧酶降解的关系[J]. 植物生理学报. 2000, 26 (6): 481-486.
[24]	姜绮霞, 袁洪. 组蛋白乙酰转移酶及脱乙酰基酶的作用及调节机制[J]. 生命的化学. 2007, 27 (3): 218-220.

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Interacting Proteins of Tamarix hispida Translation Initiation Factor 1A and Their Expression Pattern Analysis

1. State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, Heilongjiang, China

Received Date: 2014-04-15

Abstract: Five different proteins which interacted with Tamarix hispida translation initiation factor (TheIF1A) gene were obtained by yeast two-hybrid assay. They were RNA polymerase beta II subunit, ATP synthase CF1 alpha subunit protein, cytochrome b6/f complex subunit IV, ribulose 1, 5-bisphosphate carboxylase/oxygenase small subunit and histone acetyltransferase. Furthermore, the expression patterns of these proteins and TheIF1A under normal and abiotic stresses were analyzed by RT-PCR analysis. The results showed that these interacting proteins were all shared similar expression patterns with TheIF1A, suggesting that these proteins may act together to regulate the stress tolerance of T. hispida.

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Reference (24)

[1]	Holcik M, N. Sonenberg. Translational Control in Stress and Apoptosis[J]. Nature Reviews Molecular Cell Biology. 2005, 6 (4): 318-327.
[2]	Howarth C. Molecular Responses of Plants to an Increased Incidence of Heat Shock[J]. Plant, Cell & Environment. 1991, 14 (8): 831-841.
[3]	Dong Z, Zhang J. Initiation Factor eIF3 and Regulation of mRNA Translation, Cell Growth, and Cancer[J]. Critical Reviews in Oncology/Hematology. 2006, 59 (3): 169-180.
[4]	Pyronnet S, Sonenberg N. Cell-Cycle-Dependent Translational Control[J]. Current Opinion in Genetics & Development. 2001, 11 (1): 13-18.
[5]	Kyrpides N C, Woese C R. Universally Conserved Translation Initiation Factors[J]. Proceedings of the National Academy of Sciences. 1998, 95 (1): 224-228.
[6]	Mecak A R, Shin B S, Dever T E, et al. Engaging the Ribosome: Universal IFs of Translation[J]. Trends in Biochemical Sciences. 2001, 26 (12): 705-709.
[7]	Olsen D S, Savner E M, Mathew A, et al. Domains of eIF1A That Mediate Binding to eIF2, eIF3 and eIF5b and Promote Ternary Complex Recruitment in Vivo[J]. The EMBO Journal. 2003, 22 (2): 193-204.
[8]	Rausell A, Kanhonou R, Yenush L, et al. The Translation Initiation Factor eIF1A Is an Important Determinant in the Tolerance to NaCl Stress in Yeast and Plants[J]. The Plant Journal. 2003, 34 (3): 257-267.
[9]	王留强, 柽柳真核起始因子 5A (elFSA) 基因的功能研究 [D].东北林业大学硕士论文. 2011.
[10]	Taylor D N, Carr JP. The Gcd10 Subunit of Yeast eIF-3 Binds the Methyltransferase-Like Domain of the 126 and 183 Kda Replicase Proteins of Tobacco Mosaic Virus in the Yeast Two-Hybrid System[J]. Journal of General Virology. 2000, 81 (6): 1587-1591.
[11]	Stebbins-Boaz B, Cao Q, de Moor C H, et al. Maskin Is a CPEB-Associated Factor That Transiently Interacts with eIF-4e[J]. Molecular Cell. 1999, 4 (6): 1017-1027.
[12]	于丽丽. 柽柳 ThDREB 和 TheIF5A 基因抗逆功能研究 [D]. 东北林业大学, 2011.
[13]	管蕊. 利用酵母双杂交系统筛选与 BMNPVPE38 和 VIF-1 相互作用的蛋白 [D]. 江苏科技大学, 2012.
[14]	Livak K J, Schmittgen T D. Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2^-ΔΔC_T Method[J]. Methods (San Diego, Calif.). 2001, 25 (4): 402-408.
[15]	Battiste J L, Pestova T V, Hellen C U, et al. The eIF1A Solution Structure Reveals a Large RNA-Binding Surface Important for Scanning Function[J]. Molecular Cell. 2000, 5 (1): 109-119.
[16]	Chaudhuri J, Si K, Maitra U. Function of Eukaryotic Translation Initiation Factor 1A (eIF1A) (Formerly Called eIF-4c) in Initiation of Protein Synthesis[J]. Journal of Biological Chemistry. 1997, 272 (12): 7883-7891.
[17]	Tezara W, Mitchell V, Driscoll S, et al. Water Stress Inhibits Plant Photosynthesis by Decreasing Coupling Factor and ATP[J]. Nature. 1999, 401 (6756): 914-917.
[18]	Niu X, Bressan RA, Hasegawa P M,et al. Ion Homeostasis in NaCl Stress Environments[J]. Plant Physiology. 1995, 109 (3): 735.
[19]	Wang W, Vinocur B, Altman A. Plant Responses to Drought, Salinity and Extreme Temperatures: Towards Genetic Engineering for stress tolerance[J]. Planta. 2003, 218 (1): 1-14.
[20]	毛海滨, 李国富, 阮翔, 等. 质体库和细胞色素 B6/f 参与调控蓝细菌 (Synechocystis Sp. Pcc 6803) 的状态转换[J]. Acta Biophysica Sinica. 2002, 18 (3).
[21]	施定基, 张超, 李世明, 等. 蓝藻与植物叶绿体光合系统基因的生物信息学研究[J]. 遗传学报. 2004, 31 (6): 627-633.
[22]	梅杨, 李海蓝, 谢晋, 等. 核酮糖-1, 5-二磷酸羧化酶/加氧酶 (Rubisco)[J]. 植物生理学通讯. 2007, 43 (2): 363-368.
[23]	刘拥海, 彭新湘, 李明启. 水稻叶片中过氧化氢与核酮糖-1, 5-二磷酸羧化酶/加氧酶降解的关系[J]. 植物生理学报. 2000, 26 (6): 481-486.
[24]	姜绮霞, 袁洪. 组蛋白乙酰转移酶及脱乙酰基酶的作用及调节机制[J]. 生命的化学. 2007, 27 (3): 218-220.

Interacting Proteins of Tamarix hispida Translation Initiation Factor 1A and Their Expression Pattern Analysis

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Interacting Proteins of Tamarix hispida Translation Initiation Factor 1A and Their Expression Pattern Analysis

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