Cloning and Expression Analysis of PsCCH from Populus simonii under Stresses of Heavy Metal
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1.
State Key Laboratory of Forest Genetics and Tree Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091, China
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Received Date:
2013-02-26
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Abstract
The CCH gene from Populus simonii leaf was cloned according to a strategy combining bioinformatics and molecular biological approaches and its expression in response to heavy metals and plant growth regulators was detected by qPCR. This gene encodes a polypeptide of 85 amino acids, named as PsCCH. The deduced amino acid sequence of PsCCH possesses the conserved metal-binding motif in its N-terminal (MXCXXC) and has the classic βαββαβ-fold structure. Phylogeny analysis showed that the PsCCH was much closer to the CCH from Hevea brasiliensis,Jatropha curcas and Vitis vinifera (with 90%-93% in amino acids similarity). The results of qPCR showed that the expression of PsCCH was regulated by various heavy metals and plant growth regulators: the PsCCH transcripts increased within one hour and then decreased to a lower level after 3 hours, such as in zinc, aluminum, low copper and jasmonic acid treatments; and the PsCCH transcripts continuously decreased during the treatment, such as in high copper, cobalt, mercury and salicylic acid treatments.
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