Cloning and SNP Analysis of 1-Deoxy-D-xylulose-5-phosphate Reductoisomerases DXR) in Litsea cubeba

LIU Ying-guan; WU Qing-ke; HE Guan-shun; WANG Yang-dong; YANG Su-su; CHEN Yi-cun; GAO Ming

Based on the results of transcriptome sequencing, the DXR gene cDNA was isolated from the flower bud of Litsea cubeba by the method of RT-PCR. At the same time, full-length sequence of DXR was first obtained from the genomic DNA by PCR and splice, and named as LcDXR. Sequence analysis showed that the full-length cDNA of LcDXR was 1 501bp, including 5' non-coding region 34bp and 3'non-coding region 53bp and encoded 470 amino acids. The theoretical molecular weight of LcDXR was 51.12kd and the isoelectric point was predicted as 6.62. The full-length gene was 12 601bp with 12 exons and 11 introns. Single nucleotide polymorphic (SNP) sites of LcDXR from 10 sources were analyzed, the results showed that there were 10 SNPs within cDNA region, and 4 SNPs lead to amino acids changes. Point mutation was analyzed by Swiss-PDB Viewer to find fine structure changes resulted by amino acids mutations. The Lys119Thr mutation in Anyuan, Jiangxi Province was shown to have H-bonds change, which may have an impact on the enzyme activity.

1. Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang 311400, Zhejiang, China

2. Forestry Bureau of Tianlin County Guangxi Zhuang Autonomous Region, Tianlin 533300, Guangxi, China

Received Date: 2014-09-02

Abstract: Based on the results of transcriptome sequencing, the DXR gene cDNA was isolated from the flower bud of Litsea cubeba by the method of RT-PCR. At the same time, full-length sequence of DXR was first obtained from the genomic DNA by PCR and splice, and named as LcDXR. Sequence analysis showed that the full-length cDNA of LcDXR was 1 501bp, including 5' non-coding region 34bp and 3'non-coding region 53bp and encoded 470 amino acids. The theoretical molecular weight of LcDXR was 51.12kd and the isoelectric point was predicted as 6.62. The full-length gene was 12 601bp with 12 exons and 11 introns. Single nucleotide polymorphic (SNP) sites of LcDXR from 10 sources were analyzed, the results showed that there were 10 SNPs within cDNA region, and 4 SNPs lead to amino acids changes. Point mutation was analyzed by Swiss-PDB Viewer to find fine structure changes resulted by amino acids mutations. The Lys119Thr mutation in Anyuan, Jiangxi Province was shown to have H-bonds change, which may have an impact on the enzyme activity.

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Reference (20)

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Cloning and SNP Analysis of 1-Deoxy-D-xylulose-5-phosphate Reductoisomerases DXR) in Litsea cubeba

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