Storage Condition and Viability Change of Camellia japonica Pollen

JIA Wen-qing; WANG Shao-ping; FAN Zheng-qi; LI Ji-yuan

Volume 28 Issue 3

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Storage Condition and Viability Change of Camellia japonica Pollen

1.
Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou 311400, Zhejiang, China
2.
School of Horticulture Landscape Architecture, He'nan Institute of Science and Technology, Xinxiang 453003, He'nan, China

Received Date: 2014-07-28

Abstract

Fresh pollen of Camellia japonica was used to test the pollen viability by in vitro culture. The effects of different store methods on pollen viability and the activity of SOD, POD and CAT were also investigated. The results show that 1.The best combination for pollen germination was 150 g·L-1 sucrose, 0.05 g·L-1 boric acid, 100 mg·L-1 GA3, and 900 g·L-1 Ca(NO3)2. 2.-80℃ sealed storage was the best storage condition to keep the vitality of the pollen. 3.After 360 days' storage, the activity of the three kinds of enzymes, the antioxidant capacity and aging ability of the pollen kept high. 4.The germination proportion of pollen decreased significantly when the activities of SOD, POD and CAT were around the highest levels. 5.The three enzymes had different responses to the storage temperature. POD and CAT were responding protection enzymes at room temperature, while SOD was a responding protection enzyme at 4℃, however. CAT was a responding protection enzyme at -20℃ and -80℃. The effect of three kinds of enzymes on pollen germination are in the order of SOD>POD>CAT.
- Camellia japonica,
- pollen,
- germination,
- storage,
- SOD,
- POD,
- CAT

References

[1]	中国树木志编委会. 中国树木志: 第2册[M].北京: 中国林业出版社, 1994:1602-1603.
[2]	王翔, 刘庆华, 王奎玲, 等. 耐冬山茶(Camellia, japonica L.)花粉活力和柱头可授性研究[J].西南农业学报, 2008, 21(4):1078-1080.
[3]	杨成利, 刘庆华, 王奎玲, 等. 耐冬山茶(Camellia japonica L.)花粉活力测定及人工授粉研究[J].江西农业学报, 2009, 21(8):70-72.
[4]	潘瑞炽. 植物生理学[M]. 北京: 高等教育出版社, 2001:257-261.
[5]	贾文庆, 尤扬, 刘会超, 等. 矮牡丹花粉形态观察与萌发特性研究[J]. 西北林学院学报, 2012, 27(5):76-79.
[6]	管雨, 贾文庆, 刘会超, 等. 木瓜花粉生活力测定及储藏特性[J]. 浙江农林大学学报, 2012, 29(5):790-794.
[7]	刘艳萍, 朱延林, 马永涛, 等. 不同贮藏条件对紫玉兰花粉保护酶活性及萌发率的影响[J]. 东北林业大学学报, 2013, 41(4):59-61.
[8]	谭健晖. 贮藏温度和时间对马尾松花粉保护酶活性及萌发率的影响[J]. 林业科学, 2011, 47(9):28-32.
[9]	赵文飞, 邢世岩, 姜永旭, 等. 贮藏时间对银杏花粉保护酶活性和萌发率的影响[J]. 武汉植物学研究, 2004, 22(3):259-263.
[10]	李合生, 孙群, 赵世杰, 等. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2000:164-167.
[11]	Omran R G. Peroxide levels and the activities of catalase, peroxidase and indoleacetic acid oxidase during and after chilling cucumber seedlings[J]. Plant Physiology, 1980, 65(2):407-408.
[12]	许林, 杜克兵, 陈法志, 等. 川鄂连蕊茶花粉的形态、生活力及贮藏力研究[J]. 园艺学报, 2010, 37(11):1857-1862.
[13]	杨盛美, 宋维希, 唐一春, 等. 茶组植物花粉生活力测定及种间杂交研究[J]. 中国农学通报, 2010, 26(8):115-118.
[14]	许林, 李应发, 周忠诚, 等. 两种茶树花粉的生活力及贮藏力研究[J]. 湖北农业科学, 2013, 52(24):6067-6071.
[15]	黄永芳, 吴雪辉, 何美儿, 等. 3种油茶植物花粉贮藏及生活力的研究[J]. 福建林学院学报, 2011, 31(1):56-59.
[16]	张涛, 黄敏. 蔗糖和PEG对山茶花花粉离体萌发的影响[J]. 北方园艺, 2009, 34(1):101-102.
[17]	Miltiadis V, Porlingis I C. Effect of temperature on pollen germination and pollen tube growth, effective pollination period and fruit set of pear [J]. Hort Science, 1985, 20(4):733-735.
[18]	律春燕, 王雁, 朱向涛, 等. 黄牡丹花粉生活力测定方法比较研究[J]. 林业科学研究, 2010, 23(2):272-277.
[19]	刘会超, 贾文庆, 尤扬, 等. 红花酢浆草花粉萌发及贮藏特性研究[J]. 江西农业大学学报, 2010, 32(1):185-189.
[20]	贾文庆, 尤扬, 刘会超, 等. 鸢尾花粉生活力测定及贮藏特性研究[J]. 西北农业学报, 2012, 21(8):182-185.
[21]	程伟, 赵志刚, 曾杰, 等. 西南桦花粉离体萌发的适宜条件与萌发特征[J]. 林业科学研究, 2007, 20(2):209-212.
[22]	郭蓓, 史芳芳, 李晨, 等. 20 个荷花品种花粉活力测定及贮藏研究[J]. 河南科学, 2014, 32(1):29-32.
[23]	Kanazaw S, Sano S, Koshiba T, et al.Changes in antioxidative in cucumber cotyledons during naturalsence:comparison with those during dark-induced senescence[J]. Physiologia Plantarum, 2000, 109(2):211-216.
[24]	Giannopolitis C N, Ries S K.Superoxide dismutase: purification and quantitative relationship with water-soluble protein in seedlings[J]. P1ant Physiology, 1977, 59(2):3l5-318.

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Storage Condition and Viability Change of Camellia japonica Pollen

1. Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou 311400, Zhejiang, China

2. School of Horticulture Landscape Architecture, He'nan Institute of Science and Technology, Xinxiang 453003, He'nan, China

Received Date: 2014-07-28

Abstract: Fresh pollen of Camellia japonica was used to test the pollen viability by in vitro culture. The effects of different store methods on pollen viability and the activity of SOD, POD and CAT were also investigated. The results show that 1.The best combination for pollen germination was 150 g·L-1 sucrose, 0.05 g·L-1 boric acid, 100 mg·L-1 GA3, and 900 g·L-1 Ca(NO3)2. 2.-80℃ sealed storage was the best storage condition to keep the vitality of the pollen. 3.After 360 days' storage, the activity of the three kinds of enzymes, the antioxidant capacity and aging ability of the pollen kept high. 4.The germination proportion of pollen decreased significantly when the activities of SOD, POD and CAT were around the highest levels. 5.The three enzymes had different responses to the storage temperature. POD and CAT were responding protection enzymes at room temperature, while SOD was a responding protection enzyme at 4℃, however. CAT was a responding protection enzyme at -20℃ and -80℃. The effect of three kinds of enzymes on pollen germination are in the order of SOD>POD>CAT.

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Reference (24)

[1]	中国树木志编委会. 中国树木志: 第2册[M].北京: 中国林业出版社, 1994:1602-1603.
[2]	王翔, 刘庆华, 王奎玲, 等. 耐冬山茶(Camellia, japonica L.)花粉活力和柱头可授性研究[J].西南农业学报, 2008, 21(4):1078-1080.
[3]	杨成利, 刘庆华, 王奎玲, 等. 耐冬山茶(Camellia japonica L.)花粉活力测定及人工授粉研究[J].江西农业学报, 2009, 21(8):70-72.
[4]	潘瑞炽. 植物生理学[M]. 北京: 高等教育出版社, 2001:257-261.
[5]	贾文庆, 尤扬, 刘会超, 等. 矮牡丹花粉形态观察与萌发特性研究[J]. 西北林学院学报, 2012, 27(5):76-79.
[6]	管雨, 贾文庆, 刘会超, 等. 木瓜花粉生活力测定及储藏特性[J]. 浙江农林大学学报, 2012, 29(5):790-794.
[7]	刘艳萍, 朱延林, 马永涛, 等. 不同贮藏条件对紫玉兰花粉保护酶活性及萌发率的影响[J]. 东北林业大学学报, 2013, 41(4):59-61.
[8]	谭健晖. 贮藏温度和时间对马尾松花粉保护酶活性及萌发率的影响[J]. 林业科学, 2011, 47(9):28-32.
[9]	赵文飞, 邢世岩, 姜永旭, 等. 贮藏时间对银杏花粉保护酶活性和萌发率的影响[J]. 武汉植物学研究, 2004, 22(3):259-263.
[10]	李合生, 孙群, 赵世杰, 等. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2000:164-167.
[11]	Omran R G. Peroxide levels and the activities of catalase, peroxidase and indoleacetic acid oxidase during and after chilling cucumber seedlings[J]. Plant Physiology, 1980, 65(2):407-408.
[12]	许林, 杜克兵, 陈法志, 等. 川鄂连蕊茶花粉的形态、生活力及贮藏力研究[J]. 园艺学报, 2010, 37(11):1857-1862.
[13]	杨盛美, 宋维希, 唐一春, 等. 茶组植物花粉生活力测定及种间杂交研究[J]. 中国农学通报, 2010, 26(8):115-118.
[14]	许林, 李应发, 周忠诚, 等. 两种茶树花粉的生活力及贮藏力研究[J]. 湖北农业科学, 2013, 52(24):6067-6071.
[15]	黄永芳, 吴雪辉, 何美儿, 等. 3种油茶植物花粉贮藏及生活力的研究[J]. 福建林学院学报, 2011, 31(1):56-59.
[16]	张涛, 黄敏. 蔗糖和PEG对山茶花花粉离体萌发的影响[J]. 北方园艺, 2009, 34(1):101-102.
[17]	Miltiadis V, Porlingis I C. Effect of temperature on pollen germination and pollen tube growth, effective pollination period and fruit set of pear [J]. Hort Science, 1985, 20(4):733-735.
[18]	律春燕, 王雁, 朱向涛, 等. 黄牡丹花粉生活力测定方法比较研究[J]. 林业科学研究, 2010, 23(2):272-277.
[19]	刘会超, 贾文庆, 尤扬, 等. 红花酢浆草花粉萌发及贮藏特性研究[J]. 江西农业大学学报, 2010, 32(1):185-189.
[20]	贾文庆, 尤扬, 刘会超, 等. 鸢尾花粉生活力测定及贮藏特性研究[J]. 西北农业学报, 2012, 21(8):182-185.
[21]	程伟, 赵志刚, 曾杰, 等. 西南桦花粉离体萌发的适宜条件与萌发特征[J]. 林业科学研究, 2007, 20(2):209-212.
[22]	郭蓓, 史芳芳, 李晨, 等. 20 个荷花品种花粉活力测定及贮藏研究[J]. 河南科学, 2014, 32(1):29-32.
[23]	Kanazaw S, Sano S, Koshiba T, et al.Changes in antioxidative in cucumber cotyledons during naturalsence:comparison with those during dark-induced senescence[J]. Physiologia Plantarum, 2000, 109(2):211-216.
[24]	Giannopolitis C N, Ries S K.Superoxide dismutase: purification and quantitative relationship with water-soluble protein in seedlings[J]. P1ant Physiology, 1977, 59(2):3l5-318.

Storage Condition and Viability Change of Camellia japonica Pollen

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通讯作者: 陈斌, bchen63@163.com

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Storage Condition and Viability Change of Camellia japonica Pollen

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