Analysis on Antioxidant Activities in vitro of Refined Powder of Phyllanthus emblica L.

LI Xian; GAN Jin; ZHANG Wen-wen; HOU Bin; ZHANG Hong; FENG Ying

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Analysis on Antioxidant Activities in vitro of Refined Powder of Phyllanthus emblica L.

1.
Research Institute of Resources Insects, Chinese Academy of Forestry
2.
Laboratory of Cultivation and Utilization of Resources Insects, State Forestry Administration, Kunming 650224, Yunnan, China

Received Date: 2015-05-15

Abstract

The antioxidant activities in vitro of refined powder of Phyllanthus emblica L. were detected by methods based on chemical measurement and cell modeling. The results of anti-peroxidation ability and iron reduction ability trials showed that the refined powder of P. emblica L. had strong ability of anti-peroxidation, and it increased with the increasing of concentration with a clear dose-response relationship. When the concentration reached 800 μg·mL-1, the ability of anti-peroxidation was almost equal to the positive control VC with the same concentration. Meanwhile, it also had iron reduction ability that accompanied by significant dose-response relationship. By in vitro cell culture method, H2O2 was used to induce SH-SY5Y cells damages to establish the cell model of oxidative damage. The author detected the effect of different concentrations of powder on cell survival rate for the model cells by MTT assay. The results indicated that refined powder could show better protective effect to oxidative damaged SH-SY5Y cells in the range of 600 1 200 μg·mL-1. When the concentration reached 1 200 μg·mL-1, the cell survival rate was the highest and refined powder exhibited the best antioxidant activity. Both methods proved the strong antioxidant activities of refined powder of P. emblica L. in vitro.
- refined powder,
- Phyllanthus emblica,
- antioxidant activities in vitro,
- FRAP assay,
- SH-SY5Y cells

References

[1]	药典委员会. 中华人民共和国药典:一部[M]. 北京:中国医药科技出版社, 2010:167.
[2]	徐义侠. 余甘子化学成分及总酚提取工艺研究[D]. 北京:北京中医药大学, 2009.
[3]	张晓梅, 刘晓芳, 高云涛. 余甘子提取物抗氧化作用研究[J]. 微量元素与健康究, 2011,28(3):29-30.
[4]	李秀丽, 叶峰, 俞腾飞. 余甘子的药理研究进展[J]. 时珍国医国药, 2006,17(2):266-267.
[5]	Jose JK, Kuttan G, Kuttan R. Antitumour activity of Emblica officinalis[J]. Journal of Ethnopharmacology, 2001, 75(2-3):65-69.
[6]	Jose JK, Kuttan R.Hepatoprotective activity of Emblica officinalis and Chyavanaprash[J]. Journal Of Ethnopharmacology, 2000, 72(1-2):135-140.
[7]	刘晓丽, 赵谋明. 余甘子果汁活性成分与抗氧化活性研究[J]. 食品与发酵工业, 2006,32(5):151-154.
[8]	张雯雯, 郑华, 冯颖,等. 正交试验优化余甘子果汁微胶囊化工艺[J]. 食品科学, 2015, 36(2):25-29.
[9]	ZHENG Hua, ZHANG Hong, GAN Jin, et al. Technique of spray-drying on Phyllanthus emblica L. and anti-oxidation of the product[J]. Advanced Materials Research, 2012, 524/525/526/527:2321-2324.
[10]	Nakashima A,Ohtawa M,Masuda N, et al. Antioxidative effects of fluvastatin, and its major metabolites[J]. Yakμgaku Zasshi, 2001,121(1):113-116.
[11]	Benzie I F, Strain J J. The Ferric Reducing Ability of Plasma (FRAP) as a Measure of "Antioxidant Power":The FRAP Assay[J]. Analytical biochemistry, 1996, 239(1):70-76.
[12]	王世博, 邱景富, 白群华, 等. 黄芪甲苷对H₂O₂致PC12细胞氧化应激损伤的保护作用[J].中国药理学通报, 2011,27(11):1603-1609.
[13]	高蒙蒙, 孙桂波, 斯建勇, 等. 红车轴草总黄酮对H₂O₂诱导的血管内皮细胞损伤的保护作用[J]. 中国药理学通报, 2013, 29(2):201-207.
[14]	Roessner A,Kuester D,Malfertheiner P,et al. Oxidative stress in ulcerative colitis-associated carcinogenesis[J]. Pathology-Research and practice, 2008,204(7):511-524.
[15]	Peter C, Wootton-Beard, Lisa Ryan. Improving public health?:The role of antioxidant-rich fruit and vegetable beverages[J]. Food Research International,2011,44(10):3135-3148.
[16]	张献忠, 黄海智, 钟烈洲, 等. 植物提取物体外抗氧化活性评价方法研究进展[J]. 中国粮油学报, 2012,27(11):122-128.
[17]	Blair IA. DNA adducts with lipid peroxidation products[J]. Journal of Biological Chemistry, 2008,283(23):15545-15549.
[18]	阿萨. 白藜芦醇通过调节抗氧化酶活性而呈现抗肿瘤效应[D]. 湖南:中南大学, 2013.
[19]	崔炳权, 林元藻. 余甘子果汁的营养成分分析[J]. 食品工业科技, 2007,28(8):222-223.
[20]	刘晓丽, 吴克刚, 柴向华, 等. 余甘子多酚作为食用油脂抗氧化剂的研究[J]. 中国食品添加剂, 2010(3):194-198.
[21]	肖湘, 俞丽君, 邱玉莹, 等. 油柑多糖的提取与清除氧自由基作用的研究[J]. 中国药学杂志, 1998, 33(5):279-281.
[22]	Bendich A. The Antioxidant Role of Vitamine C[J]. Adv. in Free Racical Biology & Medicine, 1986, 2:419-444.
[23]	孙静. LMWH-SOD对神经细胞保护作用及其机理研究[D]. 山东:山东大学,2005.
[24]	阎林茂, 郭宇龙, 张延龙, 等. 百合花瓣酚类物质及其抗氧化活性的分析[J]. 食品科学, 2013,34(7):51-55.

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Analysis on Antioxidant Activities in vitro of Refined Powder of Phyllanthus emblica L.

1. Research Institute of Resources Insects, Chinese Academy of Forestry

2. Laboratory of Cultivation and Utilization of Resources Insects, State Forestry Administration, Kunming 650224, Yunnan, China

Received Date: 2015-05-15

Abstract: The antioxidant activities in vitro of refined powder of Phyllanthus emblica L. were detected by methods based on chemical measurement and cell modeling. The results of anti-peroxidation ability and iron reduction ability trials showed that the refined powder of P. emblica L. had strong ability of anti-peroxidation, and it increased with the increasing of concentration with a clear dose-response relationship. When the concentration reached 800 μg·mL-1, the ability of anti-peroxidation was almost equal to the positive control VC with the same concentration. Meanwhile, it also had iron reduction ability that accompanied by significant dose-response relationship. By in vitro cell culture method, H2O2 was used to induce SH-SY5Y cells damages to establish the cell model of oxidative damage. The author detected the effect of different concentrations of powder on cell survival rate for the model cells by MTT assay. The results indicated that refined powder could show better protective effect to oxidative damaged SH-SY5Y cells in the range of 600 1 200 μg·mL-1. When the concentration reached 1 200 μg·mL-1, the cell survival rate was the highest and refined powder exhibited the best antioxidant activity. Both methods proved the strong antioxidant activities of refined powder of P. emblica L. in vitro.

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Reference (24)

[1]	药典委员会. 中华人民共和国药典:一部[M]. 北京:中国医药科技出版社, 2010:167.
[2]	徐义侠. 余甘子化学成分及总酚提取工艺研究[D]. 北京:北京中医药大学, 2009.
[3]	张晓梅, 刘晓芳, 高云涛. 余甘子提取物抗氧化作用研究[J]. 微量元素与健康究, 2011,28(3):29-30.
[4]	李秀丽, 叶峰, 俞腾飞. 余甘子的药理研究进展[J]. 时珍国医国药, 2006,17(2):266-267.
[5]	Jose JK, Kuttan G, Kuttan R. Antitumour activity of Emblica officinalis[J]. Journal of Ethnopharmacology, 2001, 75(2-3):65-69.
[6]	Jose JK, Kuttan R.Hepatoprotective activity of Emblica officinalis and Chyavanaprash[J]. Journal Of Ethnopharmacology, 2000, 72(1-2):135-140.
[7]	刘晓丽, 赵谋明. 余甘子果汁活性成分与抗氧化活性研究[J]. 食品与发酵工业, 2006,32(5):151-154.
[8]	张雯雯, 郑华, 冯颖,等. 正交试验优化余甘子果汁微胶囊化工艺[J]. 食品科学, 2015, 36(2):25-29.
[9]	ZHENG Hua, ZHANG Hong, GAN Jin, et al. Technique of spray-drying on Phyllanthus emblica L. and anti-oxidation of the product[J]. Advanced Materials Research, 2012, 524/525/526/527:2321-2324.
[10]	Nakashima A,Ohtawa M,Masuda N, et al. Antioxidative effects of fluvastatin, and its major metabolites[J]. Yakμgaku Zasshi, 2001,121(1):113-116.
[11]	Benzie I F, Strain J J. The Ferric Reducing Ability of Plasma (FRAP) as a Measure of "Antioxidant Power":The FRAP Assay[J]. Analytical biochemistry, 1996, 239(1):70-76.
[12]	王世博, 邱景富, 白群华, 等. 黄芪甲苷对H₂O₂致PC12细胞氧化应激损伤的保护作用[J].中国药理学通报, 2011,27(11):1603-1609.
[13]	高蒙蒙, 孙桂波, 斯建勇, 等. 红车轴草总黄酮对H₂O₂诱导的血管内皮细胞损伤的保护作用[J]. 中国药理学通报, 2013, 29(2):201-207.
[14]	Roessner A,Kuester D,Malfertheiner P,et al. Oxidative stress in ulcerative colitis-associated carcinogenesis[J]. Pathology-Research and practice, 2008,204(7):511-524.
[15]	Peter C, Wootton-Beard, Lisa Ryan. Improving public health?:The role of antioxidant-rich fruit and vegetable beverages[J]. Food Research International,2011,44(10):3135-3148.
[16]	张献忠, 黄海智, 钟烈洲, 等. 植物提取物体外抗氧化活性评价方法研究进展[J]. 中国粮油学报, 2012,27(11):122-128.
[17]	Blair IA. DNA adducts with lipid peroxidation products[J]. Journal of Biological Chemistry, 2008,283(23):15545-15549.
[18]	阿萨. 白藜芦醇通过调节抗氧化酶活性而呈现抗肿瘤效应[D]. 湖南:中南大学, 2013.
[19]	崔炳权, 林元藻. 余甘子果汁的营养成分分析[J]. 食品工业科技, 2007,28(8):222-223.
[20]	刘晓丽, 吴克刚, 柴向华, 等. 余甘子多酚作为食用油脂抗氧化剂的研究[J]. 中国食品添加剂, 2010(3):194-198.
[21]	肖湘, 俞丽君, 邱玉莹, 等. 油柑多糖的提取与清除氧自由基作用的研究[J]. 中国药学杂志, 1998, 33(5):279-281.
[22]	Bendich A. The Antioxidant Role of Vitamine C[J]. Adv. in Free Racical Biology & Medicine, 1986, 2:419-444.
[23]	孙静. LMWH-SOD对神经细胞保护作用及其机理研究[D]. 山东:山东大学,2005.
[24]	阎林茂, 郭宇龙, 张延龙, 等. 百合花瓣酚类物质及其抗氧化活性的分析[J]. 食品科学, 2013,34(7):51-55.

Analysis on Antioxidant Activities in vitro of Refined Powder of Phyllanthus emblica L.

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通讯作者: 陈斌, bchen63@163.com

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Analysis on Antioxidant Activities in vitro of Refined Powder of Phyllanthus emblica L.

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