Analysis on Leaf Morphology and Photosynthesis Physiology of Polyploidized Populus yunnanensis Seedlings

TANG Jun-rong; LI Bin; ZHU Li-na; ZHANG Jun; HE Cheng-zhong; LI Xian-zhong; XIN Pei-yao

Volume 29 Issue 1

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Analysis on Leaf Morphology and Photosynthesis Physiology of Polyploidized Populus yunnanensis Seedlings

1.
Yunnan Provincial Key Laboratory for Forest Disaster Warning and Control, Southwest Forestry University, Kunming 650224, Yunnan, China
2.
Key Laboratory of Biodiversity Conservation in Southwest Region of China, State Forestry Administration, Southwest Forestry University, Kunming 650224, Yunnan, China
3.
Faculty of Life Science, Southwest Forestry University, Kunming 650224, Yunnan, China
4.
Yunnan Forestry Senior Technical School, Kunming 650213, Yunnan, China

Received Date: 2015-06-15

Abstract

[Objective]The morphological and photo-physioligical difference caused by genome dosage of different ploidies Populus yunnanensis were investigated, in order to provide theoretical guidance for P.yunnanensis polyploid breeding.[Method]Polyploid P. yunnanensis seedlings were obtained which were used as materials to determine the leaf size, leaf thickness, palisade tissue thickness, stomata characteristics and chlorophyll content compared with diploid P. yunnanensis. [Result]The results showed that all the leaf sizes of mosaic and tetraploid were larger than diploid. The leaf thicknesses of mosaic and tetraploid were 1.16 times and 1.41 times of that of the diploid, while the palisade tissues of mosaic and tetraploid were 1.10 times and 1.23 times thicker than that of the diploid. The stomata number of polyploidized leaf surface in per unit visula field was obviously fewer than that of the diploid, but the volum was larger than that of the diploid. The total chlorophyll contents in mosaic and tetraploid were respectively 127% and 141% of that of diploid. The net photosynthesis ratios of mosaic and tetraploid were faster than that of the diploid, while the stomatal conductance and transpiration rate of diploid were little different from the diploid, and the intercellular CO2 concentration in the diurnal variation process did not change significantly in these materials.[Conclusion]The obvious genetic dose-effect was put up after the chromosome number increased. The theoretical basis and practical guidance were offered by the research and the important material foundation were provided for polyploidized P. yunnanensis clone as well.
- Pinus yunnanensis,
- polyploidy,
- genetic effect,
- analysis

References

[1]	中国科学院中国植物志编辑委员会.中国植物志[M].北京:科学出版社,1984.
[2]	何承忠,张志毅,陈宝昆,等.滇杨遗传改良策略初论[J].西部林业科学,2004,33(1):44-48.
[3]	康向阳,王君.杨树多倍体诱导技术研究[M].北京:科学出版社,2010.
[4]	罗向东,戴亮芳,钱春桃,等.黄瓜属不同倍性异源多倍体的形态及生理特性分析[J].西北植物学报,2008, 28(3):542-546.
[5]	Dhooghe E, Van L K, Eeckhaut T,et al. Mitotic chromosome doubling of plant tissues in vitro[J].Plant Cell, Tissue and Organ Culture,2011,104(3):359-373.
[6]	李云.杨树三倍体选育研究进展[J].植物学通报,2001,18(4):451-458.
[7]	刘晓静,华小平,陆波,等.彩色马蹄莲品种'Parfait'多倍体诱导及其生物学特征变化[J].植物资源与环境学报,2014,23(3):81-85,101.
[8]	Tang Z Q, Chen D L, Song Z J,et al. In vitro induction and identification of tetraploid plants of Paulownia tomentosa[J]. Plant Cell, Tissue and Organ Culture,2010,102(2):213-220.
[9]	Saikat G, Nirmal M, Somnath B, et al. Induction and identification of tetraploids using in vitro colchicine treatment of Gerbera jamesonii Bolus cv. Sciella[J].Plant Cell, Tissue and OrganCulture,2011,106(3):485-493.
[10]	白凤莹,曾青青,康宁,等.毛白杨基因库优树倍性检测及性状对比分析[J].北京林业大学学报,2015,37(4):113-119.
[11]	王君.青杨派树种多倍体诱导技术研究[D].北京:北京林业大学,2009.
[12]	石乐,张平冬,毛彦科,等.高温处理诱导染色体加倍获得白杨杂种多倍体[J].核农学报,2012,26(8):1118-1123.
[13]	陈杰,周军,孙正海,等.组织培养结合秋水仙素诱导滇杨多倍体的研究[J].云南农业大学学报:自然科学版,2013,28(2):251-256.
[14]	辛培尧,陈杰,唐军荣,等.滇杨多倍体的诱导研究[J].中南林业科技大学学报:自然科学版,2014, 34(3): 24-28,57.
[15]	李贇.果树多倍体倍性鉴定方法的研究[D].泰安:山东农业大学,1997.
[16]	王侠剑.桑树多倍体植株育成初报[J].蚕业科学,1981,7(2):83-86.
[17]	房桂干,邓拥军,李萍.三倍体毛白杨制浆性能的评价[J].林业科技管理,2001(增刊): 87-90.
[18]	李凤兰,张志毅,张民侠.白杨染色体加倍技术研究及三倍体育种(Ⅲ)-加倍体某些形态特征的观察[J].北京林业大学学报,1994,16(2): 15-18.
[19]	胡晓丽.三倍体毛白杨纸浆材新品种产业化应用关键技术研究[D].北京:北京林业大学,2006.
[20]	魏跃,王开冻,颜志明,等.矮牵牛的多倍体诱导试验[J].浙江农业科学,2007(2):164-166.
[21]	Beck S L, Dunlop R W, Fossey A. Stomatal length and frequency as a measure of ploidy level in black wattle, Acacia mearnsii (de Wild)[J]. Bot J Linn Soc, 2003,141(2):177-181.
[22]	Aryavand A, Ehdaie B, Tran B, et al. Stomatal frequency and size differentiate ploidy levels in Aegilops neglecta[J]. Genet Resour Crop Evol,2003,50(2):175-182.
[23]	Shiga I, Uno Y, Kanechi M, et al. Identification of polyploidy of in vitro anther-derived shoots of Asparagus officinalis L. by flow cytometric analysis and measurement of stomatal length[J]. Journal of the Japanese Society for Horticultural Science, 2009,78(1):103-108.
[24]	戴洪义.葡萄的染色体倍性与气孔性状的关系及其判别分析[J].葡萄栽培与酿酒,1990(2): 5-9.
[25]	高彩云,潘惠新,贾恒,等.美洲黑杨×小叶杨杂种多倍体诱导研究[J].西北植物学报,2010, 30(1):50-55.
[26]	漆慧娟. 甜叶菊多倍体诱导及其生物学特性的研究[D]. 临安:浙江农林大学, 2014.
[27]	郭巧萍. 桃叶型半夏多倍体诱导研究[D]. 杭州:浙江理工大学, 2009.
[28]	刘庆忠,刘朋,赵红军,等.同源四倍体皇家嘎啦苹果的生物学及光合生理特性研究[J].中国农业科学,2002,35(12):1573-1578.
[29]	施先锋.西瓜多倍体诱导及倍性鉴定的研究[D].武汉:华中农业大学,2007.

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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Analysis on Leaf Morphology and Photosynthesis Physiology of Polyploidized Populus yunnanensis Seedlings

1. Yunnan Provincial Key Laboratory for Forest Disaster Warning and Control, Southwest Forestry University, Kunming 650224, Yunnan, China

2. Key Laboratory of Biodiversity Conservation in Southwest Region of China, State Forestry Administration, Southwest Forestry University, Kunming 650224, Yunnan, China

3. Faculty of Life Science, Southwest Forestry University, Kunming 650224, Yunnan, China

4. Yunnan Forestry Senior Technical School, Kunming 650213, Yunnan, China

Received Date: 2015-06-15

Abstract: [Objective]The morphological and photo-physioligical difference caused by genome dosage of different ploidies Populus yunnanensis were investigated, in order to provide theoretical guidance for P.yunnanensis polyploid breeding.[Method]Polyploid P. yunnanensis seedlings were obtained which were used as materials to determine the leaf size, leaf thickness, palisade tissue thickness, stomata characteristics and chlorophyll content compared with diploid P. yunnanensis. [Result]The results showed that all the leaf sizes of mosaic and tetraploid were larger than diploid. The leaf thicknesses of mosaic and tetraploid were 1.16 times and 1.41 times of that of the diploid, while the palisade tissues of mosaic and tetraploid were 1.10 times and 1.23 times thicker than that of the diploid. The stomata number of polyploidized leaf surface in per unit visula field was obviously fewer than that of the diploid, but the volum was larger than that of the diploid. The total chlorophyll contents in mosaic and tetraploid were respectively 127% and 141% of that of diploid. The net photosynthesis ratios of mosaic and tetraploid were faster than that of the diploid, while the stomatal conductance and transpiration rate of diploid were little different from the diploid, and the intercellular CO2 concentration in the diurnal variation process did not change significantly in these materials.[Conclusion]The obvious genetic dose-effect was put up after the chromosome number increased. The theoretical basis and practical guidance were offered by the research and the important material foundation were provided for polyploidized P. yunnanensis clone as well.

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Reference (29)

[1]	中国科学院中国植物志编辑委员会.中国植物志[M].北京:科学出版社,1984.
[2]	何承忠,张志毅,陈宝昆,等.滇杨遗传改良策略初论[J].西部林业科学,2004,33(1):44-48.
[3]	康向阳,王君.杨树多倍体诱导技术研究[M].北京:科学出版社,2010.
[4]	罗向东,戴亮芳,钱春桃,等.黄瓜属不同倍性异源多倍体的形态及生理特性分析[J].西北植物学报,2008, 28(3):542-546.
[5]	Dhooghe E, Van L K, Eeckhaut T,et al. Mitotic chromosome doubling of plant tissues in vitro[J].Plant Cell, Tissue and Organ Culture,2011,104(3):359-373.
[6]	李云.杨树三倍体选育研究进展[J].植物学通报,2001,18(4):451-458.
[7]	刘晓静,华小平,陆波,等.彩色马蹄莲品种'Parfait'多倍体诱导及其生物学特征变化[J].植物资源与环境学报,2014,23(3):81-85,101.
[8]	Tang Z Q, Chen D L, Song Z J,et al. In vitro induction and identification of tetraploid plants of Paulownia tomentosa[J]. Plant Cell, Tissue and Organ Culture,2010,102(2):213-220.
[9]	Saikat G, Nirmal M, Somnath B, et al. Induction and identification of tetraploids using in vitro colchicine treatment of Gerbera jamesonii Bolus cv. Sciella[J].Plant Cell, Tissue and OrganCulture,2011,106(3):485-493.
[10]	白凤莹,曾青青,康宁,等.毛白杨基因库优树倍性检测及性状对比分析[J].北京林业大学学报,2015,37(4):113-119.
[11]	王君.青杨派树种多倍体诱导技术研究[D].北京:北京林业大学,2009.
[12]	石乐,张平冬,毛彦科,等.高温处理诱导染色体加倍获得白杨杂种多倍体[J].核农学报,2012,26(8):1118-1123.
[13]	陈杰,周军,孙正海,等.组织培养结合秋水仙素诱导滇杨多倍体的研究[J].云南农业大学学报:自然科学版,2013,28(2):251-256.
[14]	辛培尧,陈杰,唐军荣,等.滇杨多倍体的诱导研究[J].中南林业科技大学学报:自然科学版,2014, 34(3): 24-28,57.
[15]	李贇.果树多倍体倍性鉴定方法的研究[D].泰安:山东农业大学,1997.
[16]	王侠剑.桑树多倍体植株育成初报[J].蚕业科学,1981,7(2):83-86.
[17]	房桂干,邓拥军,李萍.三倍体毛白杨制浆性能的评价[J].林业科技管理,2001(增刊): 87-90.
[18]	李凤兰,张志毅,张民侠.白杨染色体加倍技术研究及三倍体育种(Ⅲ)-加倍体某些形态特征的观察[J].北京林业大学学报,1994,16(2): 15-18.
[19]	胡晓丽.三倍体毛白杨纸浆材新品种产业化应用关键技术研究[D].北京:北京林业大学,2006.
[20]	魏跃,王开冻,颜志明,等.矮牵牛的多倍体诱导试验[J].浙江农业科学,2007(2):164-166.
[21]	Beck S L, Dunlop R W, Fossey A. Stomatal length and frequency as a measure of ploidy level in black wattle, Acacia mearnsii (de Wild)[J]. Bot J Linn Soc, 2003,141(2):177-181.
[22]	Aryavand A, Ehdaie B, Tran B, et al. Stomatal frequency and size differentiate ploidy levels in Aegilops neglecta[J]. Genet Resour Crop Evol,2003,50(2):175-182.
[23]	Shiga I, Uno Y, Kanechi M, et al. Identification of polyploidy of in vitro anther-derived shoots of Asparagus officinalis L. by flow cytometric analysis and measurement of stomatal length[J]. Journal of the Japanese Society for Horticultural Science, 2009,78(1):103-108.
[24]	戴洪义.葡萄的染色体倍性与气孔性状的关系及其判别分析[J].葡萄栽培与酿酒,1990(2): 5-9.
[25]	高彩云,潘惠新,贾恒,等.美洲黑杨×小叶杨杂种多倍体诱导研究[J].西北植物学报,2010, 30(1):50-55.
[26]	漆慧娟. 甜叶菊多倍体诱导及其生物学特性的研究[D]. 临安:浙江农林大学, 2014.
[27]	郭巧萍. 桃叶型半夏多倍体诱导研究[D]. 杭州:浙江理工大学, 2009.
[28]	刘庆忠,刘朋,赵红军,等.同源四倍体皇家嘎啦苹果的生物学及光合生理特性研究[J].中国农业科学,2002,35(12):1573-1578.
[29]	施先锋.西瓜多倍体诱导及倍性鉴定的研究[D].武汉:华中农业大学,2007.

Analysis on Leaf Morphology and Photosynthesis Physiology of Polyploidized Populus yunnanensis Seedlings

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