Technique of Tissue Culture of Acacia cincinnata

[Objective] To establish the technique system of tissue culture for 16-year-old Acacia cincinnata elite tree. [Method] Newborn branches with axillary buds from 16-year-old elite tree were collected as explants to study the sterilization strategy, primary culture, multiplication culture, rooting and transplant of A. cincinnata. [Result] A cutting orchard was established by using the newborn branches of 16-year-old A. cincinnata elite trees as cuttings. It was proved that the middle part of stem segments collected from the orchard was the best. As sterilized with 75% alcohol for 0.5 min and 0.1% HgCl2 for 18 min, the survival rate and shooting rate of explants were 69.33% and 86.67% respectively; the best primary culture medium was modify MS+ sucrose 40 g·L-1, with germination rate 91.33%; the highest multiplication index was 3.50 after 35 d cultured on MS+6-BA 0.5 mg·L-1+NAA 0.1 mg·L-1+sucrose 30 g·L-1; the best medium for rooting was 1/2MS+IBA 0.25 mg·L-1+ NAA 0.5 mg·L-1+ sucrose 40 g·L-1 with rooting rate 96.11% after cultured 15 d. The survival rate reached 71.11% after being transplanted the rooted plantlets to nutrition cup with sand. [Conclusion] This study would help to resolve the problems of sterilization and shoot induction of mature A. cincinnata elite tree, and the tissue culture technique could contribute to A. cincinnata breeding program and large-scale seedling propagation.